Nowak M, Madej J A, Dziegiel P, Kanzawa H
Department of Pathological Anatomy, Pathophysiology, Microbiology and Forensic Veterinary Medicine, Faculty of Veterinary Medicine, Agricultural Academy, ul. Norwida 31, 50-375 Wrocław, Poland.
Pol J Vet Sci. 2006;9(1):57-62.
The studies aimed at identification of neoplastic cells at the S phase of mitotic cycle in mammary gland adenocarcinomas of bitches. The material was sampled from bitches of various races, aging 6 to 12 years, in which the mammary gland tumours developed spontaneously. The tumours were verified histopathologically and, then, immunohistochemical reactions were performed in order to detect cells which had incorporated BrdU (bromodeoxyuridine), contained Ki-67 or PCNA antigen. The histological preparations were photographed and obtained pictures were subjected to computer-assisted image analysis using Axiophot microscope (Carl Zeiss) coupled to a computer and the Multi-ScaneBase V 8.08 software, working under Windows. Fifty percent of sections from mammary gland adenocarcinomas demonstrated BrdU labelling index of 4-5%, 40% of 1-3%, while in the remaining 10% of examined tumours no BrdU incorporation could be demonstrated. No evident relationship could be detected between the presence of BrdU incorporation and Ki-67 or PCNA antigen presence but a significant correlation was demonstrated between the expression of Ki-67 and PCNA.
这些研究旨在鉴定母犬乳腺腺癌有丝分裂周期S期的肿瘤细胞。材料取自6至12岁不同品种的自发发生乳腺肿瘤的母犬。对肿瘤进行组织病理学验证，然后进行免疫组化反应，以检测掺入BrdU（溴脱氧尿苷）、含有Ki-67或PCNA抗原的细胞。对组织学切片进行拍照，使用与计算机相连的Axiophot显微镜（卡尔·蔡司）和在Windows系统下运行的Multi-ScaneBase V 8.08软件对获得的图片进行计算机辅助图像分析。50%的乳腺腺癌切片显示BrdU标记指数为4 - 5%，40%为1 - 3%，而在其余10%的检测肿瘤中未发现BrdU掺入。在BrdU掺入的存在与Ki-67或PCNA抗原的存在之间未检测到明显关系，但Ki-67和PCNA的表达之间存在显著相关性。
