Igarashi M, Komiya Y, Kurokawa M
FEBS Lett. 1985 Nov 18;192(2):239-42. doi: 10.1016/0014-5793(85)80115-0.
N-Acetyl-D-[6-3H]mannosamine was injected into the 9th dorsal root ganglion of Xenopus laevis and the intra-axonal transport of chloroform/methanol-soluble radioactivity was analyzed using thin-layer chromatography coupled with fluorography. Three radioactive groups were distinct in consecutive segments of the sciatic nerve. The first is due to N-acetyl-D-mannosamine itself which labels the nerve uniformly, but does not seem to migrate within axons. The second group, representing most probably CMP-sialic acid, migrates at about 8 mm/day at 15 degrees C. The third is a species of ganglioside uniquely present in the frog nerve, and this migrates at 1-3 mm/day. Our demonstration of the intra-axonal transport of CMP-sialic acid affords direct support to the contention that sialosylation of the ganglioside can occur in axon terminals.
将N-乙酰-D-[6-³H]甘露糖胺注射到非洲爪蟾的第九背根神经节中，并使用薄层色谱结合荧光自显影技术分析氯仿/甲醇可溶性放射性物质的轴突内运输。在坐骨神经的连续节段中，有三个放射性基团明显不同。第一个是由于N-乙酰-D-甘露糖胺本身，它均匀地标记神经，但似乎不在轴突内迁移。第二组，最有可能代表CMP-唾液酸，在15摄氏度下以约8毫米/天的速度迁移。第三组是青蛙神经中独特存在的一种神经节苷脂，其迁移速度为1-3毫米/天。我们对CMP-唾液酸轴突内运输的证明为神经节苷脂的唾液酸化可发生在轴突终末这一论点提供了直接支持。
