从cDNA核苷酸序列推导的兔肺子宫珠蛋白一级结构
Primary structure of rabbit lung uteroglobin as deduced from the nucleotide sequence of a cDNA.
作者信息
López de Haro M S, Nieto A
出版信息
FEBS Lett. 1985 Dec 2;193(2):247-9. doi: 10.1016/0014-5793(85)80162-9.
PMID:2415398
Abstract
Double-stranded cDNA was synthesized from partially purified uteroglobin mRNA from rabbit lung. A cDNA coding for lung uteroglobin was then cloned in the plasmid pUC18 and both the nucleotide sequence and the derived amino acid sequence were determined. This allowed us to demonstrate unequivocally that uteroglobins from lung and uterus are identical proteins.
摘要
双链互补脱氧核糖核酸（cDNA）由兔肺中部分纯化的子宫珠蛋白信使核糖核酸（mRNA）合成。然后，将编码肺子宫珠蛋白的cDNA克隆到质粒pUC18中，并确定了核苷酸序列和推导的氨基酸序列。这使我们能够明确证明，来自肺和子宫的子宫珠蛋白是相同的蛋白质。
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