1] Chinese Academy of Sciences Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology No.11, First North Road, Zhongguancun, Beijing 100190, P.R. China [2] College of Life Science and Bioengineering Beijing University of Technology No.100 Pingleyuan, Chaoyang District, Beijing 100124, P.R.China [3].
Sci Rep. 2013 Oct 24;3:3036. doi: 10.1038/srep03036.
Multifunctional nanoparticles as theranostic tools hold great potential for its unique and efficient way to visualize the process of disease treatment. However, the toxicity of conventional fluorescent labels and difficulty of functionalization limit their widespread use. Recently, a number of amino-rich polymers have demonstrated high luminescent fluorescence but rarely showed potential for in vivo imaging due to their blue fluorescence. Here, a general route has been found to construct polymer-based multifunctional nanoparticles for combined imaging and drug delivering. The weak fluorescent polyethyleneimine (PEI) has been conjugated with hydrophobic polylactide as the amphiphilic PEI for construction of nanoparticles which showed bright and multicolor fluorescence with high drug loading capacity. The paclitaxel-loaded nanoparticles showed significant therapy effect in contrast to the free paclitaxel. Meanwhile, fluorescence imaging of the nanoparticles showed accumulation around tumor. These results demonstrate a new type of polymer-based multifunctional nanoparticles for imaging-guided drug delivery.
多功能纳米粒子作为治疗诊断工具，具有独特且高效的可视化疾病治疗过程的潜力。然而，传统荧光标记物的毒性和功能化的难度限制了它们的广泛应用。最近，许多富含氨基酸的聚合物由于其呈现蓝色荧光而表现出高发光荧光，但由于其呈现蓝色荧光，很少显示出用于体内成像的潜力。在这里，我们找到了一种通用的方法来构建基于聚合物的多功能纳米粒子，用于联合成像和药物输送。弱荧光聚乙烯亚胺（PEI）与疏水性聚乳酸偶联作为两亲性 PEI 来构建纳米粒子，该纳米粒子具有高载药能力的明亮和多色荧光。载紫杉醇的纳米粒子与游离紫杉醇相比表现出显著的治疗效果。同时，纳米粒子的荧光成像显示其在肿瘤周围聚集。这些结果表明了一种新型的聚合物基多功能纳米粒子，可用于成像引导的药物输送。
