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蓖麻毒素的免疫细胞化学检测。I. 初步免疫荧光研究。

Immunocytochemical detection of ricin. I. Preliminary immunofluorescence studies.

作者信息

Cmiech H A, Morley M, Gee D J

出版信息

Histochem J. 1985 Aug;17(8):859-66. doi: 10.1007/BF01004181.

DOI:10.1007/BF01004181
PMID:2415485
Abstract

Ricin injected into rat muscle in vivo can be localized within a few hours using routine immunofluorescence techniques on formalin-fixed tissues. However, the level of sensitivity decreases with increase in size of animal injected, time after administration and decrease in dose given. These findings are discussed in relation to the known chemistry and subcellular mode of action of ricin.

摘要

使用常规免疫荧光技术对福尔马林固定的组织进行检测,在体内注射到大鼠肌肉中的蓖麻毒素可在数小时内定位。然而,随着注射动物体型的增大、给药后时间的延长以及给药剂量的减少,灵敏度水平会降低。结合蓖麻毒素已知的化学性质和亚细胞作用模式对这些发现进行了讨论。

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引用本文的文献

1
Immunocytochemical detection of ricin. II. Further studies using the immunoperoxidase method.蓖麻毒素的免疫细胞化学检测。II. 使用免疫过氧化物酶法的进一步研究。
Histochem J. 1986 Apr;18(4):189-95. doi: 10.1007/BF01676120.

本文引用的文献

1
Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro.对水痘和带状疱疹病原体在体外繁殖进行的荧光抗体研究。
Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):789-94. doi: 10.3181/00379727-86-21235.
2
Radioimmunoassays of abrin and ricin in blood.血液中相思子毒素和蓖麻毒素的放射免疫分析
J Toxicol Environ Health. 1981 Sep;8(3):409-17. doi: 10.1080/15287398109530079.
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Georgi Markov--death in a pellet.
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Cell-specific cytotoxicity expressed by a conjugate of ricin and murine monoclonal antibody directed against thy 1.1 antigen.
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Abrin and ricin: new anti-tumour substances.相思子毒素和蓖麻毒素:新型抗肿瘤物质。
Nature. 1970 Jul 18;227(5255):292-3. doi: 10.1038/227292a0.
6
Purification of galactose-binding phytoagglutinins and phytotoxin by affinity column chromatography using sepharose.使用琼脂糖通过亲和柱色谱法纯化半乳糖结合植物凝集素和植物毒素。
Experientia. 1972 Jan 15;28(1):84-5. doi: 10.1007/BF01928278.
7
Antigenicity: some molecular aspects.抗原性:一些分子层面的问题。
Science. 1969 Dec 12;166(3911):1365-74. doi: 10.1126/science.166.3911.1365.
8
Mechanism of action of the toxic lectins abrin and ricin.毒性凝集素相思子毒素和蓖麻毒素的作用机制。
Nature. 1974 Jun 14;249(458):627-31. doi: 10.1038/249627a0.
9
Ricin: the toxic protein of castor oil seeds.蓖麻毒素:蓖麻油籽中的有毒蛋白质。
Toxicology. 1974 Mar;2(1):77-102. doi: 10.1016/0300-483x(74)90044-4.
10
Treatment of abrin and ricin with -mercaptoethanol opposite effects on their toxicity in mice and their ability to inhibit protein synthesis in a cell-free system.用β-巯基乙醇处理相思子毒素和蓖麻毒素,对它们在小鼠体内的毒性以及在无细胞系统中抑制蛋白质合成的能力产生相反的影响。
FEBS Lett. 1972 Nov 15;28(1):48-50. doi: 10.1016/0014-5793(72)80674-4.