Reiss H D, Herth W
J Cell Sci. 1985 Jun;76:247-54. doi: 10.1242/jcs.76.1.247.
Pollen germination and tube growth of Lilium longiflorum in vitro are affected by 10(-5) M-nifedipine. Germinating 'tubes' form broad protuberances along the whole colpus. Short tubes show a high tendency to grow 'amoeboid-like' and to branch; or a second tube emerges in another region of the colpus. Longer tubes (greater than or equal to 100 micron) broaden irregularly or swell at their tips. The diameter of the tube can vary drastically within the same tube. With increasing time of treatment many tubes burst. Normal tip growth stops within 10 min, but protoplasmic streaming continues even after 15 h. More or less regularly, wall thickenings are formed along the whole tube or on the flanks of the germinating region after some hours. The internal calcium gradient, visualized by chlorotetracycline (CTC) fluorescence, is also disturbed. Nifedipine treatment results in uniform or irregular CTC fluorescence. Branching tubes temporarily show a new subapical CTC gradient. After 6-8 h of nifedipine treatment many cells are no longer stainable with CTC. The results indicate the presence of nifedipine-sensitive calcium channels in pollen tubes.
麝香百合花粉在体外的萌发和花粉管生长受10⁻⁵M硝苯地平的影响。萌发的“花粉管”沿整个萌发沟形成宽阔的突起。短花粉管表现出高度的“类阿米巴样”生长和分支倾向；或者在萌发沟的另一个区域出现第二条花粉管。较长的花粉管（大于或等于100微米）会不规则地变宽或在顶端肿胀。同一花粉管内的直径变化可能很大。随着处理时间的增加，许多花粉管会破裂。正常的顶端生长在10分钟内停止，但原生质流动即使在15小时后仍会继续。几小时后，或多或少会沿整个花粉管或在萌发区域的侧面形成规则的壁加厚。通过氯四环素（CTC）荧光观察到的内部钙梯度也受到干扰。硝苯地平处理导致CTC荧光均匀或不规则。分支的花粉管暂时显示出新的亚顶端CTC梯度。硝苯地平处理6-8小时后，许多细胞不再能用CTC染色。结果表明花粉管中存在对硝苯地平敏感的钙通道。
