Tsuzuki Nao, Seo Jong-pil, Yamada Kazutaka, Haneda Shingo, Furuoka Hidefumi, Tabata Yasuhiko, Sasaki Naoki
Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-city, Hokkaido, 080-8555, Japan (Tsuzuki, Seo, Yamada, Haneda, Sasaki); Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-city, Hokkaido, 080-8555, Japan (Furuoka); and Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan (Tabata).
Can Vet J. 2013 Jun;54(6):573-80.
We evaluated the curative efficacy of a gelatin β-tricalcium phosphate (β-TCP) sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2 (BMP-2), and platelet-rich plasma (PRP) by insertion into an experimentally induced osteochondral defect. A hole of 10 mm diameter and depth was drilled in the bilateral medial femoral condyles of 7 thoroughbred horses, and into each either a loaded sponge (treatment) or a saline-infused β-TCP sponge (control) was inserted. After 16 weeks, defects were examined by computed tomography, macroscopic analyses, and histological analyses. The median subchondral bone density and macroscopic subscores for joint healing were significantly higher in the treatment legs (P < 0.05). Although there was no significant difference in total histological scores between groups, hyaline cartilaginous tissue was observed across a wider area in the treatment group. Equine joint healing can be enhanced by inserting a BMP-2-, MSC-, and PRP-impregnated β-TCP sponge at the lesion site.
我们通过将负载间充质干细胞（MSC）、骨形态发生蛋白-2（BMP-2）和富血小板血浆（PRP）的明胶β-磷酸三钙（β-TCP）海绵植入实验诱导的骨软骨缺损处，评估了其治疗效果。在7匹纯种马的双侧股骨内侧髁上钻一个直径10毫米、深度相同的孔，然后在每个孔中分别植入负载海绵（治疗组）或注入生理盐水的β-TCP海绵（对照组）。16周后，通过计算机断层扫描、宏观分析和组织学分析对缺损进行检查。治疗组的软骨下骨密度中位数和关节愈合的宏观评分显著更高（P < 0.05）。虽然两组之间的组织学总评分没有显著差异，但在治疗组中观察到透明软骨组织覆盖的区域更广泛。在病变部位插入BMP-2、MSC和PRP浸渍的β-TCP海绵可以促进马关节的愈合。
