微通道中生物保存溶液的干燥动力学
Desiccation kinetics of biopreservation solutions in microchannels.
作者信息
Aksan Alptekin, Irimia Daniel, He Xiaoming, Toner Mehmet
出版信息
J Appl Phys. 2006;99(6):2181280. doi: 10.1063/1.2181280.
Abstract
A microfluidic device was utilized to measure the viscosity gradients formed in carbohydrate solutions of biological significance during desiccation and skin formation. A complementary numerical model employed the free volume theory to predict the concentration-dependent diffusion coefficients and viscosity gradients in concentrated solutions. It was established that the glassy skin formation at the gas-liquid interface played a key role in water entrapment and the formation and persistence of very steep concentration and viscosity gradients in the desiccating solutions. The results of this study highlighted an important phenomenon that should be accounted for during isothermal drying of glass-forming solutions: solutions with high glass transition temperatures, inevitably, dry heterogeneously. In the final product, there are significant spatial variations in water and solute content affecting the storage stability.
摘要
利用微流控装置测量了具有生物学意义的碳水化合物溶液在干燥和形成皮膜过程中形成的粘度梯度。一个补充性的数值模型采用自由体积理论来预测浓溶液中浓度依赖的扩散系数和粘度梯度。研究确定，气液界面处玻璃态皮膜的形成在截留水分以及干燥溶液中形成并持续存在非常陡峭的浓度和粘度梯度方面起着关键作用。本研究结果突出了一个在玻璃形成溶液等温干燥过程中应予以考虑的重要现象：具有高玻璃化转变温度的溶液不可避免地会不均匀干燥。在最终产品中，水和溶质含量存在显著的空间变化，影响储存稳定性。
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