Dávila Soledad, Vignola María Belén, Cremonezzi David, Simes Juan C, Soriano Fernando, Campana Vilma R
Cátedra de Física Biomédica. Facultad de Ciencias Médicas. Universidad Nacional de Córdoba. Santa Rosa 1085. (5000) Córdoba, Argentina.
Laser Ther. 2011;20(4):287-92. doi: 10.5978/islsm.11-or-02.
The aim of the present work was to study the effect of Helium-Neon (HeNe) and Gallium Arsenide (GaAs) laser upon nitric oxide (NO) plasma levels, an inflammatory biomarker associated with oxidative stress, in rats with experimental myopathy. These were evaluated through histological assessment.
The groups studied were: (A) control (intact rats that received LLLT sham exposures), (B) rats with myopathy and sacrificed at 24 h later, (C) rats with myopathy and sacrificed 8 days later, (D) rats with myopathy and treated with HeNe laser, (E) rats with myopathy and treated with GaAs laser, (F) intact rats treated with HeNe laser and (G) intact rats treated with GaAs laser. Myopathy was induced by injecting 50μl of 1% carrageenan λ (type IV) in the left gastrocnemius muscle. Low Level Laser Therapy (LLLT) was applied with 9.5 J.cm(-2) daily for 10 consecutive days with each laser. The determination of the NO was made by spectrophotometry. The muscles were stained with Hematoxylin-Eosin and examined by optic microscopy. Quantitative variables were statistically analyzed by the Fisher test, and categorical by applying Pearson's Chi Squared test at p <0.05 for all cases.
In groups B and C, NO was significantly increased compared to groups A, D, E, F and G (p<0.05). In group C, the percentage of area with inflammatory infiltration was significantly increased compared to the other groups (p<0.001).
LLLT decreased plasma levels of NO in rats with experimental myopathies and significant muscle recovery.
本研究旨在探讨氦氖（HeNe）激光和砷化镓（GaAs）激光对实验性肌病大鼠一氧化氮（NO）血浆水平的影响，NO是一种与氧化应激相关的炎症生物标志物。通过组织学评估对其进行评价。
所研究的组包括：（A）对照组（接受低强度激光疗法假照射的完整大鼠），（B）肌病大鼠，24小时后处死，（C）肌病大鼠，8天后处死，（D）肌病大鼠并用HeNe激光治疗，（E）肌病大鼠并用GaAs激光治疗，（F）用HeNe激光治疗的完整大鼠，（G）用GaAs激光治疗的完整大鼠。通过在左腓肠肌注射50μl 1%的卡拉胶λ（IV型）诱导肌病。每种激光均连续10天每天以9.5 J.cm(-2)的剂量进行低强度激光疗法（LLLT）。通过分光光度法测定NO。肌肉用苏木精-伊红染色并通过光学显微镜检查。所有情况下，定量变量通过Fisher检验进行统计学分析，分类变量通过应用Pearson卡方检验进行分析，p<0.05。
与A、D、E、F和G组相比，B组和C组的NO显著升高（p<0.05）。与其他组相比，C组炎症浸润面积百分比显著增加（p<0.001）。
低强度激光疗法降低了实验性肌病大鼠的NO血浆水平，并实现了显著的肌肉恢复。
