Silva Igor Henrique Morais, de Andrade Samantha Cardoso, de Faria Andreza Barkokebas Santos, Fonsêca Deborah Daniela Diniz, Gueiros Luiz Alcino Monteiro, Carvalho Alessandra Albuquerque Tavares, da Silva Wylla Tatiana Ferreira, de Castro Raul Manhães, Leão Jair Carneiro
Department of Clinic and Preventive Dentistry, Pernambuco Federal University, Av. Prof. Moraes Rego, 1235, Recife, PE, Brazil, 50670-901.
Department of Nutrition, Pernambuco Federal University, Recife, PE, Brazil.
Lasers Med Sci. 2016 Dec;31(9):1855-1862. doi: 10.1007/s10103-016-2061-1. Epub 2016 Sep 16.
The aim of this study was to evaluate the influence of low-level laser therapy (LLLT) with different parameters and wavelengths on nitric oxide (NO) release and cell viability. Irradiation was performed with Ga-Al-As laser, continuous mode and wavelengths of 660 and 808 nm at different energy and power densities. For each wavelength, powers of 30, 50, and 100 mW and times of 10, 30, and 60 s were used. NO release was measured using Griess reaction, and cell viability was evaluated by mitochondrial reduction of bromide 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to formazan. LLLT promoted statistically significant changes in NO release and MTT value only at the wavelength of 660 nm (p < 0.05). LLLT also promoted an increase in the NO release and cell viability when the energy densities 64 (p = 0.04) and 214 J/cm (p = 0.012), respectively, were used. LLLT has a significant impact on NO release without affecting cell viability, but the significance of these findings in the inflammatory response needs to be further studied.
本研究的目的是评估不同参数和波长的低强度激光疗法（LLLT）对一氧化氮（NO）释放和细胞活力的影响。使用Ga-Al-As激光进行照射，采用连续模式，波长为660和808 nm，能量和功率密度不同。对于每个波长，分别使用30、50和100 mW的功率以及10、30和60 s的时间。使用格里斯反应测量NO释放，并通过溴化3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）线粒体还原为甲臜来评估细胞活力。仅在660 nm波长处，LLLT促进了NO释放和MTT值的统计学显著变化（p < 0.05）。当分别使用能量密度64（p = 0.04）和214 J/cm（p = 0.012）时，LLLT还促进了NO释放和细胞活力的增加。LLLT对NO释放有显著影响，而不影响细胞活力，但这些发现对炎症反应的意义需要进一步研究。
