Department of Medical Biology and Genetic, Süleyman Demirel University School of Medicine, Isparta, Turkey.
J Breast Cancer. 2013 Sep;16(3):285-90. doi: 10.4048/jbc.2013.16.3.285. Epub 2013 Sep 30.
The aim of this study was to evaluate and compare the rate of sister chromatid exchange (SCE), the occurrence of micronuclei, and the lymphocyte proliferation rate index (PRI) in patients with breast cancer, their first-degree relatives, and healthy volunteers.
We analyzed the frequency of SCE and micronuclei, and the PRI in the peripheral blood lymphocytes of 30 women with breast cancer, 22 of their female family members, and 20 age-matched healthy female volunteers.
SCE occurred significantly more often in the lymphocytes of breast cancer patients (10.84±0.4 per metaphase), compared with their first-degree relatives (7.45±0.54) and controls (5.94±0.2) (p<0.001 for both). The mean SCE frequency was not statistically different between first-degree relatives and controls (p=0.071). Similarly, micronuclei occurred at a significantly higher rate in breast cancer patients (9.6±0.72), and in their first-degree relatives (7±0.64), compared to controls (3.85±0.4) (p<0.001 and p=0.001, respectively). There was also a significant difference between the occurrence of micronuclei in patients compared to their family members (p=0.021). The PRI was significantly lower in patients (1.61±0.1), compared with both their first-degree relatives (1.75±0.1), and controls (1.74±0.1) (p=0.001 and p=0.002, respectively).
Increased SCE and the occurrence of micronuclei, as well as a reduced PRI are associated with breast cancer. Furthermore, increased SCE and the frequency of micronuclei in a first-degree relative suggest that they exhibit greater genetic instability than women of the same age.
本研究旨在评估和比较乳腺癌患者、其一级亲属和健康志愿者的姐妹染色单体交换（SCE）率、微核发生率和淋巴细胞增殖率指数（PRI）。
我们分析了 30 名乳腺癌女性、22 名女性一级亲属和 20 名年龄匹配的健康女性志愿者外周血淋巴细胞中的 SCE 和微核频率以及 PRI。
乳腺癌患者的淋巴细胞中 SCE 发生频率明显高于一级亲属（10.84±0.4/每中期）和对照组（5.94±0.2/每中期）（均<0.001）。一级亲属和对照组之间 SCE 频率的平均值无统计学差异（p=0.071）。同样，乳腺癌患者（9.6±0.72）和一级亲属（7±0.64）的微核发生率明显高于对照组（3.85±0.4）（均<0.001）。患者微核发生率与一级亲属之间也存在显著差异（p=0.021）。患者的 PRI 明显低于一级亲属（1.61±0.1）和对照组（1.74±0.1）（均<0.001）。
SCE 增加、微核发生以及 PRI 降低与乳腺癌相关。此外，一级亲属中 SCE 增加和微核频率提示其遗传不稳定性高于同年龄女性。
