Dhillon V S, Kler R S, Dhillon I K
Department of Human Genetics, Guru Nanak Dev University, Amritsar, India.
Cancer Genet Cytogenet. 1996 Jan;86(1):54-7. doi: 10.1016/0165-4608(95)00168-9.
Cytogenetic studies have been carried out using the G-banding technique in peripheral blood lymphocytes of 14 patients with carcinoma of the cervix uteri. Simultaneously, sister chromatid exchange (SCE) was also analyzed in the peripheral blood lymphocytes of these patients, along with those of 20 age-matched control subjects. The frequency of aberrant metaphases is significantly higher in patients with carcinoma of the cervix uteri (7.85%) than in the age-matched controls (3.35%). A large number of chromosome aberrations in lymphocytes of these patients have also been detected. Sister chromatid exchange (SCE) was also analyzed in lymphocytes of 14 patients with carcinoma of the cervix uteri and 20 age-matched control subjects. The mean SCE frequencies were 9.44 +/- 0.34 (n = 637) and 6.09 +/- 0.24 (n = 900) per metaphase in patients and controls, respectively. The increase of SCE frequency in cancer patients was statistically significant (p < 0.001), but not seen in controls. Our results suggest that patients with carcinoma of the cervix uteri show a degree of chromosomal instability that might be related to a predisposition to neoplasia.
已使用G显带技术对14例子宫颈癌患者的外周血淋巴细胞进行了细胞遗传学研究。同时，还对这些患者以及20名年龄匹配的对照者的外周血淋巴细胞进行了姐妹染色单体交换（SCE）分析。子宫颈癌患者中异常中期的频率（7.85%）显著高于年龄匹配的对照者（3.35%）。还检测到这些患者淋巴细胞中的大量染色体畸变。对14例子宫颈癌患者和20名年龄匹配的对照者的淋巴细胞也进行了姐妹染色单体交换（SCE）分析。患者和对照者每个中期的平均SCE频率分别为9.44±0.34（n = 637）和6.09±0.24（n = 900）。癌症患者中SCE频率的增加具有统计学意义（p < 0.001），但在对照者中未观察到。我们的结果表明，子宫颈癌患者表现出一定程度的染色体不稳定性，这可能与肿瘤易感性有关。
