Kieu K, Mehravar S, Gowda R, Norwood R A, Peyghambarian N
College of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.
Biomed Opt Express. 2013 Sep 17;4(10):2187-95. doi: 10.1364/BOE.4.002187. eCollection 2013.
We demonstrate label-free multi-photon imaging of biological samples using a compact Er(3+)-doped femtosecond fiber laser mode-locked by a single-walled carbon nanotube (CNT). These compact and low cost lasers have been developed by various groups but they have not been exploited for multiphoton microscopy. Here, it is shown that various multiphoton imaging modalities (e.g. second harmonic generation (SHG), third harmonic generation (THG), two-photon excitation fluorescence (TPEF), and three-photon excitation fluorescence (3PEF)) can be effectively performed on various biological samples using a compact handheld CNT mode-locked femtosecond fiber laser operating in the telecommunication window near 1560nm. We also show for the first time that chlorophyll fluorescence in plant leaves and diatoms can be observed using 1560nm laser excitation via three-photon absorption.
我们展示了使用由单壁碳纳米管（CNT）锁模的紧凑型掺铒（Er(3+)）飞秒光纤激光器对生物样品进行无标记多光子成像。这些紧凑且低成本的激光器已由多个研究小组开发出来，但尚未用于多光子显微镜。在此，结果表明，使用工作在1560nm附近电信窗口的紧凑型手持式CNT锁模飞秒光纤激光器，可在各种生物样品上有效地实现各种多光子成像模式（例如二次谐波产生（SHG）、三次谐波产生（THG）、双光子激发荧光（TPEF）和三光子激发荧光（3PEF））。我们还首次表明，通过三光子吸收，利用1560nm激光激发可以观察到植物叶片和硅藻中的叶绿素荧光。
