Katsumata Y, Sato M, Tamaki K, Tsutsumi H, Yada S, Oya M
J Forensic Sci. 1985 Oct;30(4):1210-5.
A rapid and highly sensitive enzyme-linked immunosorbent assay (ELISA) for determination of human alpha-fetoprotein (AFP) using commercially available reagents was devised and applied to identification of fetal bloodstains. When experimentally prepared bloodstains, 1 by 2 mm in area, were submitted to analysis, only fetal bloodstains showed positive reactions in the present ELISA. The reactions did not change significantly when these bloodstains were stored at room temperature for one week. The present ELISA seems to be suitable for forensic science practice.
利用市售试剂设计了一种用于测定人甲胎蛋白（AFP）的快速且高灵敏度的酶联免疫吸附测定法（ELISA），并将其应用于胎儿血迹的鉴定。当对实验制备的面积为1×2毫米的血迹进行分析时，在本ELISA中只有胎儿血迹呈现阳性反应。当这些血迹在室温下保存一周时，反应没有显著变化。本ELISA似乎适用于法医学实践。
