Tsutsumi H, Okajima H, Sato K, Katsumata Y
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
J Forensic Sci. 1988 Jan;33(1):237-43.
An enzyme-linked immunosorbent assay (ELISA) of the sandwich type for identification of human urinary stains using commercially available anti-human uromucoid was developed. When experimentally prepared urinary stains of humans and animals, 2 by 2 cm in area, were subjected to analysis, human stains could be differentiated from animal ones except chimpanzee and Old World monkey ones. Stains of other human body fluids showed negative reactions. The reactions did not decrease when human urinary stains were stored at room temperature for three months. The present ELISA provides a useful presumptive test for urinary stains of human origin.
开发了一种夹心型酶联免疫吸附测定法（ELISA），用于使用市售抗人尿黏蛋白鉴定人类尿渍。当对实验制备的面积为2×2厘米的人类和动物尿渍进行分析时，除黑猩猩和旧世界猴的尿渍外，人类尿渍可与动物尿渍区分开来。其他人体体液的污渍呈阴性反应。当人类尿渍在室温下储存三个月时，反应不会减弱。本ELISA为源自人类的尿渍提供了一种有用的推定检测方法。
