Xu Tianjun, Tang Da, Jin Xiaoxiao
Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University , Zhoushan, Zhejiang Province , P.R. China.
Mitochondrial DNA. 2015 Aug;26(4):655-7. doi: 10.3109/19401736.2013.843077. Epub 2013 Oct 24.
The complete mitochondrial genome of Johnius belangerii has been determined for the first time in this article. It was 19,154 base pairs in length, and is composed of 37 genes (13 protein-coding genes, 22 tRNA genes and 2 ribosomal RNA genes). Totally, 5 notable non-coding regions were observed, and a non-coding of 1091 bp was identified as control region based on its location and AT richness. An 800 bp tandem repeat sequence was identified in the fifth non-coding region. We investigated the mitochondrial gene arrangement pattern and found that that the tRNA(Val), 12SrRNA, 16SrRNA and tRNA(Phe) genes of J. belangerii mitogenome were orderly placed at the beginning of heavy strand. This order is different from other croakers. Combine with the phylogenetic reconstruction and genes arrangement pattern of J. belangerii mitochondrial genome, we consider J. belangerii is the most ancient genus within family Sciaenidae.
本文首次测定了勒氏笛鲷的完整线粒体基因组。其长度为19,154个碱基对，由37个基因组成（13个蛋白质编码基因、22个tRNA基因和2个核糖体RNA基因）。共观察到5个显著的非编码区，其中一个1091 bp的非编码区根据其位置和富含AT的特性被鉴定为控制区。在第五个非编码区鉴定出一个800 bp的串联重复序列。我们研究了线粒体基因排列模式，发现勒氏笛鲷线粒体基因组的tRNA(Val)、12SrRNA、16SrRNA和tRNA(Phe)基因有序地位于重链起始端。这种顺序与其他笛鲷不同。结合勒氏笛鲷线粒体基因组的系统发育重建和基因排列模式，我们认为勒氏笛鲷是石首鱼科中最古老的属。
