Halfon Philippe, Sandri Maria Teresa, Raimondo Audrey, Ravet Sophie, Khiri Hacène, Sideri Mario, Penaranda Guillaume, Camus Claire, Mateos Lindemann Maria Luisa
Laboratoire Alphabio, 1 rue Melchior Guinot, Marseille 13003, France.
BMC Infect Dis. 2013 Oct 24;13:499. doi: 10.1186/1471-2334-13-499.
Cervical cancer is caused by high-risk types of human papillomavirus (HPV). DNA testing of such high-risk types of HPV could improve cervical screening.The aim of the study was to compare the sensitivities and positive predictive values of two commercially available typing assays (Qiagen LQ and Roche LA) and to comparatively assess the distribution of HPV types with these two assays.
The study population comprised 311 ASCUS + women with abnormal pap tests who were HCII positive and who were admitted to three European referral gynecology clinics between 2007 and 2010 (Madrid, Marseille and Milan). All patients underwent LQ and LA tests.
The sensitivity of the two assays for HPV typing was 94% for LQ and 99% for LA (compared with HCII). The overall concordance between LQ and LA was 93%. The three prevalent genotypes, HPV16, HPV18, and HPV31, were identified with a high concordance using the two assays: kappa 0.93, 0.83, and 0.91, respectively. Mixed genotypes were more frequently detected by LA than by LQ: 52% vs. 18%, respectively (p < .0001).
These assays have a good clinical sensitivity for detecting HPV types in CIN2+ patients and allow the virus type to be detected in the same experiment. Our study revealed no significant difference between LQ and LA for CIN2+ or CIN3+ diagnosis, indicating similar distributions of HPV types and a mixed genotype detection that is higher for LA than for LQ.
宫颈癌由高危型人乳头瘤病毒（HPV）引起。对这类高危型HPV进行DNA检测可改善宫颈癌筛查。本研究旨在比较两种市售分型检测方法（Qiagen LQ和Roche LA）的敏感性和阳性预测值，并比较评估这两种检测方法检测的HPV型别分布情况。
研究人群包括311例巴氏涂片异常的非典型鳞状细胞（ASCUS）+女性，她们HCII检测呈阳性，于2007年至2010年间被收治入欧洲三家转诊妇科诊所（马德里、马赛和米兰）。所有患者均接受了LQ和LA检测。
两种HPV分型检测方法的敏感性分别为：LQ为94%，LA为99%（与HCII相比）。LQ和LA的总体一致性为93%。使用这两种检测方法对三种常见基因型HPV16、HPV18和HPV31的检测一致性较高：kappa值分别为0.93、0.83和0.91。LA检测出的混合基因型比LQ更常见：分别为52%和18%（p <.0001）。
这些检测方法对检测CIN2+患者中的HPV型别具有良好的临床敏感性，并能在同一实验中检测病毒型别。我们的研究表明，在CIN2+或CIN3+诊断方面，LQ和LA之间无显著差异，这表明HPV型别分布相似，且LA检测出的混合基因型高于LQ。
