Department of Chemistry, Creighton University, Omaha, Nebraska, USA.
Prog Mol Biol Transl Sci. 2013;120:173-93. doi: 10.1016/B978-0-12-381286-5.00005-6.
The glmS bacterial ribozyme/riboswitch is found in a number of Gram-positive bacteria, many of which are human pathogens. Investigation of the structure and function of the glmS catalyst will aid in the development of artificial agonists/antagonists that might function as novel antibiotics. The glmS ribozyme is mechanistically unique in that it is the first RNA catalyst identified to require a coenzyme, glucosamine-6-phosphate, for RNA self-cleavage. In addition, it is the first riboswitch identified to utilize self-cleavage as a mode of genetic regulation in metabolism. Significant biochemical and biophysical data exist for the glmS ribozyme and aid in mechanistically understanding the importance of RNA and coenzyme structure to function in acid-base catalysis.
glmS 细菌核酶/核糖开关存在于许多革兰氏阳性菌中，其中许多是人类病原体。研究 glmS 催化剂的结构和功能将有助于开发可能作为新型抗生素的人工激动剂/拮抗剂。glmS 核酶在机制上是独特的，因为它是第一个被鉴定为需要辅酶葡萄糖胺-6-磷酸才能进行 RNA 自我切割的 RNA 催化剂。此外，它是第一个被鉴定为利用自我切割作为代谢中遗传调控模式的核糖开关。glmS 核酶存在大量的生化和生物物理数据，有助于在机制上理解 RNA 和辅酶结构对酸碱催化功能的重要性。
