Yeo J E, Nam B M, Yang W, Jo Y H, Lee S, Nemeno J G, Kiml B Y, Koh Y G, Lee J I
Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science & Technology (IBST), Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul, Republic of Korea; The Center for Stem Cell & Arthritis Research, Department of Orthopedic Surgery, Yonsei Sarang Hospital, Bangbae-dong, Seocho-gu, Seoul 137-060, Republic of Korea.
Transplant Proc. 2013 Oct;45(8):3122-6. doi: 10.1016/j.transproceed.2013.08.086.
The importance of drug repositioning has been gaining attention in the last few years, allowing existing pharmaceutical products to be reevaluated for potential alternative therapeutic applications. The purpose of this study was to evaluate the effects of fragmin/protamine microparticles (F/P MPs) on cell aggregates under the concept of drug repositioning.
Mesenchymal stem cells (MSCs) and embryonic rat heart-derived cardiac H9C2 cells were mixed with D-PBS, basal medium, fragmin, protamine, and F/P MPs to manufacture aggregates intended for cell transplantation. To evaluate their adhesive properties as cell carriers, we injected combinations of MSC aggregates into cartilage tissue, observing their leakage from the implantation site.
Our data demonstrated that MSCs and H9C2 cells mixed with F/P MPs rapidly produced large, viscous cellular aggregates. F/P MPs were bound to the surface of MSCs and H9C2 cells; thus, F/P MPs induced the formation of F/P MP-cell aggregates. Cell aggregates were prevented from leaking from the transplanted site.
Aggregation induced by F/P MPs may improve the efficiency of cell therapy, a novel method for transplantation.
药物重新定位的重要性在过去几年中日益受到关注，使得现有的药品能够针对潜在的替代治疗应用进行重新评估。本研究的目的是在药物重新定位的概念下评估达肝素/鱼精蛋白微粒（F/P MPs）对细胞聚集体的影响。
将间充质干细胞（MSCs）和胚胎大鼠心脏来源的心肌H9C2细胞与D - PBS、基础培养基、达肝素、鱼精蛋白和F/P MPs混合，以制备用于细胞移植的聚集体。为了评估它们作为细胞载体的黏附特性，我们将MSC聚集体的组合注射到软骨组织中，观察它们从植入部位的渗漏情况。
我们的数据表明，与F/P MPs混合的MSCs和H9C2细胞迅速产生了大的、粘性的细胞聚集体。F/P MPs结合在MSCs和H9C2细胞的表面；因此，F/P MPs诱导形成了F/P MP - 细胞聚集体。细胞聚集体被阻止从移植部位渗漏。
F/P MPs诱导的聚集可能提高细胞治疗的效率，这是一种新型的移植方法。
