Institut Pasteur, Unité de Biologie des Bactéries Pathogènes à Gram positif, Paris F-75015, France; CNRS, ERL 3526, France; Université Paris Diderot-Sorbonne Paris Cité, France.
Res Microbiol. 2013 Dec;164(10):973-8. doi: 10.1016/j.resmic.2013.09.002. Epub 2013 Oct 22.
Streptococcus gallolyticus is an emerging cause of infective endocarditis that has been epidemiologically linked to colorectal cancer. S. gallolyticus is poorly transformable using electroporation and no defined mutant has been published yet. Hence, we used mobilization to introduce plasmid DNA from Streptococcus agalactiae into S. gallolyticus using the transfer origin of the conjugative element TnGBS1 (oriTTnGBS1), followed by a classical homologous recombination technique. Two isogenic mutants of S. gallolyticus UCN34, one deleted for the pil1 pilus operon and another for the sortase A gene, were constructed and characterized. This genetic tool should help in unravelling virulence mechanisms of this bacterium.
牛链球菌是一种新兴的感染性心内膜炎病原体，与结直肠癌在流行病学上有关联。牛链球菌用电穿孔法转化效率较低，且尚未发表明确的突变体。因此，我们使用移动元件 TnGBS1（oriTTnGBS1）的转移原点将来自无乳链球菌的质粒 DNA 转入牛链球菌中，然后采用经典的同源重组技术。构建并鉴定了牛链球菌 UCN34 的两个同源突变株，一个缺失了 pil1 菌毛操纵子，另一个缺失了 sortase A 基因。该遗传工具应有助于阐明该细菌的毒力机制。
