Wewer U M, Faber M, Liotta L A, Albrechtsen R
Lab Invest. 1985 Dec;53(6):624-33.
Human decidual cells of early and late pregnancy were studied immunochemically and ultrastructurally with respect to the presence and nature of pericellular basement membrane material. The most prominent cell type in decidual tissue of both early and late pregnancy were large, mature epithelioid decidual cells (greater than 25 micron) with a distinct pericellular basement membrane, containing at least laminin, type IV collagen, heparan sulfate proteoglycan, and fibronectin. Laminin was extracted from the decidual tissue at a concentration of 3 micrograms/gm as measured by enzyme-linked immunosorbent assay. Biosynthesis of laminin was shown by [35S]methionine labeling of short term organ cultures of decidual tissue followed by immunoprecipation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and fluorography. The laminin chains migrated with the apparent molecular weights of 300 and 200 kilodaltons under reducing conditions. Two other separate populations of cells were apparent in the decidual tissue of early pregnancy. A smaller group of rounded intermediate sized (15 to 25 micron) decidual cells had focal deposits basement membrane immunoreactive material scattered at the cell surfaces. Ultrastructurally, clumps of electron dense basement membrane material were present in close vicinity to the cell membrane. Occasional populations of small (greater than 15 micron) elongated fibroblastic-like cells were identified which did not display any immunoreactivity for any of the investigated basement membrane components. The two latter populations of decidual cells were not observed in the decidual tissue investigated from the placental bed of late pregnancy. It is suggested that these three types of decidual cells might represent various stages of the sequential differentiation of stromal cells into decidual cells of the pregnant endometrium. Predecidualization of the human endometrium, which is seen in the late secretory phase of the normal menstrual cycle and in some states of hyperplasia, was also shown to be accompanied by the presence of deposits of laminin-positive material at the cell surfaces. In the latter case, these cells resembled the intermediate sized decidual cells of the pregnant endometrium. In conclusion, the results suggest that the process of decidualization and predecidualization can be characterized morphologically and immunochemically by the accumulation of basement membrane material in specific decidua cell subpopulations.
运用免疫化学和超微结构技术，研究了早孕期和晚孕期人蜕膜细胞周围基底膜物质的存在情况及性质。早孕期和晚孕期蜕膜组织中最主要的细胞类型是大型、成熟的上皮样蜕膜细胞（大于25微米），其周围有明显的基底膜，至少含有层粘连蛋白、IV型胶原、硫酸乙酰肝素蛋白聚糖和纤连蛋白。通过酶联免疫吸附测定法测得，从蜕膜组织中提取的层粘连蛋白浓度为3微克/克。通过对蜕膜组织短期器官培养物进行[35S]甲硫氨酸标记，随后进行免疫沉淀、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和荧光自显影，显示了层粘连蛋白的生物合成。在还原条件下，层粘连蛋白链的表观分子量为300和200千道尔顿。在早孕期蜕膜组织中还明显可见另外两类不同的细胞群体。一小群圆形的中等大小（15至25微米）的蜕膜细胞，其细胞表面有散在的局灶性基底膜免疫反应性物质沉积。超微结构显示，电子致密的基底膜物质团块靠近细胞膜。偶尔可识别出一些小的（大于15微米）细长的成纤维细胞样细胞，它们对所研究的任何基底膜成分均无免疫反应性。在晚孕期胎盘床的蜕膜组织中未观察到后两类蜕膜细胞群体。提示这三种类型的蜕膜细胞可能代表了基质细胞向妊娠子宫内膜蜕膜细胞顺序分化的不同阶段。在正常月经周期的分泌晚期以及某些增生状态下出现的人子宫内膜的前蜕膜化，也显示细胞表面存在层粘连蛋白阳性物质沉积。在后一种情况下，这些细胞类似于妊娠子宫内膜的中等大小的蜕膜细胞。总之，结果表明，蜕膜化和前蜕膜化过程在形态学和免疫化学上的特征是特定蜕膜细胞亚群中基底膜物质的积累。
