Wewer U M, Damjanov A, Weiss J, Liotta L A, Damjanov I
Differentiation. 1986;32(1):49-58. doi: 10.1111/j.1432-0436.1986.tb00555.x.
During mouse pregnancy, uterine stromal cells transform into morphologically distinct decidual cells under the influence of the implanting embryo and a proper hormonal environment. Mechanical stimulation of hormonally primed uterine stromal cells leads to the same morphologic alterations. The decidualization of stromal cells is characterized by the accumulation of pericellular basement-membrane material. Decidualized stromal cells of pregnancy differ in this respect from stromal cells obtained from nonpregnant uteri, which are not associated with any significant amounts of basement-membrane components. Mouse decidual cells isolated from 6- to 7-day pregnant uteri explanted in vitro continue to synthesize basement-membrane-like extracellular matrix. Using immunohistochemistry and metabolic labeling followed by immunoprecipitation, SDS-PAGE, and fluorography, it was shown that the decidual cells synthesize laminin, entactin, fibronectin, type-IV collagen, and heparan sulfate proteoglycan. Stromal cells isolated from nonpregnant uteri and explanted in vitro produced the same basement-membrane components. Apparently, these cells were stimulated by the procedure used during isolation and explanation to undergo pseudodecidualization. We thus showed that stromal cells from pregnant and nonpregnant mouse uteri synthesize significant amounts of basement-membrane components in vitro, and hence could serve as a good model for the study of normal basement-membrane components.
在小鼠妊娠期间，子宫基质细胞在着床胚胎和适当激素环境的影响下转化为形态上截然不同的蜕膜细胞。对经激素预处理的子宫基质细胞进行机械刺激会导致相同的形态学改变。基质细胞的蜕膜化特征在于细胞周围基底膜物质的积累。妊娠蜕膜化的基质细胞在这方面与从非妊娠子宫获得的基质细胞不同，后者不与任何大量的基底膜成分相关。从体外培养的妊娠6至7天的子宫中分离出的小鼠蜕膜细胞继续合成基底膜样细胞外基质。通过免疫组织化学和代谢标记，随后进行免疫沉淀、SDS-PAGE和荧光自显影，结果表明蜕膜细胞合成层粘连蛋白、巢蛋白、纤连蛋白、IV型胶原和硫酸乙酰肝素蛋白聚糖。从非妊娠子宫中分离并体外培养的基质细胞产生相同的基底膜成分。显然，这些细胞在分离和培养过程中受到刺激而发生假蜕膜化。因此，我们表明，来自妊娠和非妊娠小鼠子宫的基质细胞在体外合成大量的基底膜成分，因此可以作为研究正常基底膜成分的良好模型。
