Sun Lijun, Chen Xiwen, Jin Xingnan, Huang Qiang, Wang Weilan, Zhi Dashi, Chen Defu
Department of Neurosurgery, Tianjin Huanhu Hospital, 122 Qixiangtai Road, Tianjin, 300060, People's Republic of China,
J Mol Neurosci. 2014 Feb;52(2):294-301. doi: 10.1007/s12031-013-0144-z. Epub 2013 Oct 26.
Malignant gliomas are the most common and lethal intracranial tumors; differentiation therapy is a promising candidate for their treatment. In order to reveal the mechanisms related to glioma differentiation, after confirming that differentiation was induced by sodium phenylbutyrate in SHG-44 human glioma cells, RNA arbitrary primer differential display was used to screen differentially expressed genes. One gene was found to be upregulated by differential display, and this was also confirmed by reverse northern blot and quantitative real-time PCR analysis. After it was cloned and sequenced, the 505-bp fragment was identified as the MIBP1 (c-myc intron-binding protein 1) gene, also named Hivep2/MBP-2/Schnurri-2. Quantitative real-time PCR analysis of 30 human tissue samples revealed that the expression of MIBP1 tended to decrease with increasing WHO grade and was significantly depressed in the high malignancy gliomas group (WHO grade IV). We cloned and sequenced the MIBP1 gene, which was accepted by GenBank as number DQ231041. Finally, transfection of MIBP1 in a reverse transcription vector into glioma cells inhibited cell growth, induced differentiation, and blocked the cell cycle. Here, we identify and describe the structure and function of a differentiation-related gene, human MIBP1, in human glioma.
恶性胶质瘤是最常见且致命的颅内肿瘤；分化疗法是其治疗的一个有前景的选择。为了揭示与胶质瘤分化相关的机制，在证实苯丁酸钠可诱导SHG-44人胶质瘤细胞分化后，采用RNA任意引物差异显示技术筛选差异表达基因。通过差异显示发现一个基因上调，逆转Northern印迹和定量实时PCR分析也证实了这一点。克隆并测序后，505bp片段被鉴定为MIBP1（c-myc内含子结合蛋白1）基因，也称为Hivep2/MBP-2/Schnurri-2。对30个人类组织样本进行定量实时PCR分析显示，MIBP1的表达倾向于随着WHO分级的增加而降低，在高恶性胶质瘤组（WHO四级）中显著下调。我们克隆并测序了MIBP1基因，该基因已被GenBank收录，登录号为DQ231041。最后，将逆转录载体中的MIBP1转染到胶质瘤细胞中可抑制细胞生长、诱导分化并阻断细胞周期。在此，我们鉴定并描述了人胶质瘤中一个与分化相关的基因——人MIBP1的结构和功能。
