通过掺入硫代磷酸酯类似物对体外合成的互补DNA进行核酸酶的选择性保护。
Selective protection of in vitro synthesized cDNA against nucleases by incorporation of phosphorothioate-analogues.
作者信息
Schreiber G, Koch E M, Neubert W J
出版信息
Nucleic Acids Res. 1985 Nov 11;13(21):7663-72. doi: 10.1093/nar/13.21.7663.
The conditions for the stepwise synthesis of single- (ss) and double-stranded (ds) cDNA using thio-analogues instead of dNTPs are described in this paper. RNA of paramyxovirus Sendai (strain 6/94) serves as template in these experiments. The increased resistance of this alpha S-modified cDNA against several nucleases, like S1-Nuclease, DNase I, Exonuclease III, snake venom Phosphodiesterase (PDE) and the combination of DNase I and PDE is demonstrated.
本文描述了使用硫代类似物而非脱氧核糖核苷三磷酸（dNTPs）逐步合成单链（ss）和双链（ds）互补脱氧核糖核酸（cDNA）的条件。在这些实验中，副粘病毒仙台（6/94株）的核糖核酸（RNA）用作模板。结果表明，这种α-S修饰的互补脱氧核糖核酸（cDNA）对几种核酸酶（如S1核酸酶、脱氧核糖核酸酶I、核酸外切酶III、蛇毒磷酸二酯酶（PDE）以及脱氧核糖核酸酶I和PDE的组合）具有更高的抗性。
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