来自大肠杆菌的末端核糖腺苷酸转移酶。特性与应用。
Terminal riboadenylate transferase from Escherichia coli. Characterization and application.
作者信息
Sano H, Feix G
出版信息
Eur J Biochem. 1976 Dec 11;71(2):577-83. doi: 10.1111/j.1432-1033.1976.tb11148.x.
Abstract
Catalytic properties of terminal riboadenylate transferase from Escherichia coli and the products of the enzymic reaction were investigated. The kinetic analysis revealed that the reaction obeys the sequential ordered bi-bi mechanism. The application of conditions elaborated in this study resulted in the synthesis of products of defined size and efficient primer utilization. The tRNA(rA)n obtained was a good template for the synthesis of complementary DNA with reverse transcriptase.
摘要
对来自大肠杆菌的末端核糖腺苷酸转移酶的催化特性及其酶促反应产物进行了研究。动力学分析表明，该反应遵循有序的双底物双产物序列机制。本研究中所阐述条件的应用导致了特定大小产物的合成以及引物的高效利用。所获得的tRNA(rA)n是用逆转录酶合成互补DNA的良好模板。
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