Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina. ; Centro de Investigaciones Veterinarias Tandil, Consejo Nacional de Investigaciones Científicas y Técnicas, Tandil, Argentina.
Braz J Microbiol. 2013 Mar 19;44(1):43-9. doi: 10.1590/S1517-83822013005000003. eCollection 2013.
Meat and particularly ground beef is frequently associated with Food Poisoning episodes and breeches in Food Safety. The main goal of this research was to evaluate the bactericide effect of the probiotic Enterococcus faecalis CECT7121, against different pathogens as: Escherichia coli O157:H7, Staphylococcus aureus, Clostridium perfringens and Listeria monocytogenes, inoculated in ground beef meat. Three studies were performed to evaluate the inhibition of E. faecalis CECT7121 on ground beef meat samples inoculated with pathogens: Study I: Samples (100 g meat) were inoculated with pathogens (10(3) CFU/g)) and E. faecalis CECT7121 (10(4) CFU/g) simultaneously. Study II: Samples were inoculated with E. faecalis CECT7121 24 h before the pathogens. Study III: E. faecalis CECT7121were inoculated 24 h after pathogens. The viable counts were performed at 0, 24, 48 and 72 h post-inoculation. The simultaneous inoculation of E. faecalis CECT7121 with E. coli O157:H7 strains resulted in the absence of viable counts of bacteria at 72 h post-treatment. However, when the probiotic was added 24 h before and 24 h after the pathogen E. coli O157:H7, viable cells were not detected at 24 h and 48 h post-treatment, respectively. Consistently, neither S. aureus nor Cl. perfringens viable bacteria were detected at 48 h in whole assays when inoculated with E. faecalis CECT7121. The same trend than described before was obtained after applying the 3 models assayed for L. monocytogenes. The current assays demonstrated the bactericide activity of E. faecalis CECT7121 strain on bacterial pathogens in ground beef meat.
肉，特别是绞碎牛肉，经常与食物中毒和食品安全违规有关。本研究的主要目的是评估益生菌屎肠球菌 CECT7121 对不同病原体的杀菌效果，这些病原体包括：大肠杆菌 O157:H7、金黄色葡萄球菌、产气荚膜梭菌和单核细胞增生李斯特菌，接种到绞碎牛肉中。进行了三项研究来评估屎肠球菌 CECT7121 对接种了病原体的绞碎牛肉样本的抑制作用：研究 I：将（100 克肉）样本与病原体（10³ CFU/g）和屎肠球菌 CECT7121（10⁴ CFU/g）同时接种。研究 II：在接种病原体前 24 小时接种样本。研究 III：在接种病原体后 24 小时接种屎肠球菌 CECT7121。在接种后 0、24、48 和 72 小时进行活菌计数。屎肠球菌 CECT7121 与大肠杆菌 O157:H7 菌株同时接种导致接种后 72 小时活菌计数为零。然而，当益生菌在病原体大肠杆菌 O157:H7 之前和之后 24 小时添加时，在接种后 24 小时和 48 小时分别未检测到活细胞。同样，当接种屎肠球菌 CECT7121 时，整个试验中在 48 小时时未检测到金黄色葡萄球菌和产气荚膜梭菌的活菌。对于单核细胞增生李斯特菌，应用三种模型后也得到了与上述相同的趋势。目前的试验表明，屎肠球菌 CECT7121 菌株对绞碎牛肉中细菌病原体具有杀菌活性。
