Programa de Pós-Graduação em Processos Biotecnológicos, Universidade Federal do Paraná, Curitiba, PR, Brazil. ; Departamentos de Alimentos, Universidade Tecnológica Federal do Paraná, Campus Ponta Grossa, Ponta Grossa, PR, Brazil.
Braz J Microbiol. 2013 Apr 5;44(1):197-206. doi: 10.1590/S1517-83822013005000012. eCollection 2013.
The objective of this work is to verifying the consume of the minerals K, Na, Fe, Mg, P, S-SO4 (-2), B, N Total Kjedahl (NTK), NO3 (-)-N, and NH4 (+)-N in the production of bacterial cellulose by Acetobacter xylinum, according to the medium and the manner of cultivation. The fermentative process was in ripe and green coconut water. K and Na were determined by flame emission photometry, Mg and Fe by atomic absorption spectrophotometry, P by molecular absorption spectrophotometry, S-SO4 (-2) by barium sulphate turbidimetry, B by Azomethin-H method, NTK by Kjeldahl method, N-NO3 (-) and N-NH4 (+) by vapor distillation with magnesium oxide and Devarda's alloy, respectively. In Fermentation of ripe coconut water there were higher consumption of K (69%), Fe (84,3%), P (97,4%), S-SO2 (-2) (64,9%), B (56,1%), N-NO3 (-) (94,7%) and N-NH4 (+) (95,2%), whereas coconut water of green fruit the most consumed ions were Na (94,5%), Mg (67,7%) and NTK (56,6%). The cultivation under agitation showed higher mineral consumption. The higher bacterial cellulose production, 6 g.L(-1), was verified in the coconut water fermentative in ripe fruit, added KH2PO4, FeSO4 and NaH2PO4 kept under agitation.
本工作旨在根据培养基和培养方式，验证醋酸杆菌生产细菌纤维素时对 K、Na、Fe、Mg、P、S-SO4 (-2)、B、总凯氏氮（NTK）、NO3 (-)-N 和 NH4 (+)-N 等矿物质的消耗。发酵过程在成熟和绿色椰子水中进行。K 和 Na 通过火焰发射光度法测定，Mg 和 Fe 通过原子吸收分光光度法测定，P 通过分子吸收分光光度法测定，S-SO4 (-2) 通过硫酸钡浊度法测定，B 通过偶氮胂-H 法测定，NTK 通过凯氏定氮法测定，N-NO3 (-) 和 N-NH4 (+) 通过氧化镁和德瓦达合金的汽蒸蒸馏法分别测定。在成熟椰子水发酵中，K（69%）、Fe（84.3%）、P（97.4%）、S-SO2 (-2)（64.9%）、B（56.1%）、N-NO3 (-)（94.7%）和 N-NH4 (+)（95.2%）的消耗较高，而绿色水果椰子水中消耗较高的离子是 Na（94.5%）、Mg（67.7%）和 NTK（56.6%）。搅拌培养显示出更高的矿物质消耗。在成熟水果发酵的椰子水中，添加 KH2PO4、FeSO4 和 NaH2PO4 并保持搅拌，验证了更高的细菌纤维素产量，达到 6 g.L(-1)。
