大鼠感觉和交感神经节中 nNOS 阳性神经元的发育。
Development of nNOS-positive neurons in the rat sensory and sympathetic ganglia.
机构信息
Department of Normal Physiology and Biophysics, Yaroslavl State Medical Academy, Yaroslavl, Russia.
Department of Human Anatomy, Yaroslavl State Medical Academy, Yaroslavl, Russia.
出版信息
Neuroscience. 2014 Jan 3;256:271-81. doi: 10.1016/j.neuroscience.2013.10.013. Epub 2013 Oct 24.
Neurochemical features in sympathetic and afferent neurons are subject to change during development. Nitric oxide (NO) plays a developmental role in the nervous system. To better understand the neuroplasticity of sympathetic and afferent neurons during postnatal ontogenesis, the distribution of neuronal NO synthase (nNOS) immunoreactivity was studied in the sympathetic para- and prevertebral, nodose ganglion (NG) and Th2 and L4 dorsal root ganglia (DRG) from female Wistar rats of different ages (newborn, 10-day-old, 20-day-old, 30-day-old, 2-month-old, 6-month-old, 1-year-old, and 3-year-old). nNOS-positive neurons were revealed in all sensory ganglia but not in sympathetic ones from birth onward. The percentage of nNOS-immunoreactive (IR) neurons increased during first 10 days of life from 41.3 to 57.6 in Th2 DRG, from 40.9 to 59.1 in L4 DRG and from 31.6 to 38.5 in NG. The percentage of nNOS-IR neurons did not change in the NG later during development and senescence. However, in Th2 and L4 DRG the proportion of nNOS-IR neurons was high in animals between 10 and 30days of life and decreased up to the second month of life. In 2-month-old rats, the percentage of nNOS-IR neurons was 52.9 in Th2 DRG and 51.3 in L4 DRG. We did not find statistically significant differences in the percentage of nNOS-IR neurons between Th2 and L4 DRG and between young and aged rats. In NG and DRG of 10-day-old and older rats, a high proportion of nNOS-IR neurons binds isolectin B4. In newborn animals, only 41.3%, 45.3% and 28.4% of nNOS neuron profiles bind to IB4 in Th2, L4 DRG and NG, respectively. In 10-day-old and older rats, the number of sensory nNOS-IR neurons binding IB4 reached more than 90% in DRG and more than 80% in NG. Only a small number of nNOS-positive cells showed immunoreactivity to calcitonin gene-related peptide, neurofilament 200, calretinin. The information provided here will also serve as a basis for future studies investigating mechanisms of the development of sensory neurons.
神经化学特征在交感和传入神经元的发展过程中会发生变化。一氧化氮（NO）在神经系统的发育中起着重要作用。为了更好地理解交感和传入神经元在出生后发育过程中的神经可塑性，研究了不同年龄（新生、10 日龄、20 日龄、30 日龄、2 月龄、6 月龄、1 岁和 3 岁）雌性 Wistar 大鼠的交感副和前椎旁神经节（NG）、结状神经节（NG）和 Th2、L4 背根神经节（DRG）中的神经元型一氧化氮合酶（nNOS）免疫反应性的分布。从出生起，所有感觉神经节中都可以发现 nNOS 阳性神经元，但交感神经节中没有。nNOS 免疫反应性（IR）神经元的百分比在生命的前 10 天内从 Th2 DRG 的 41.3%增加到 57.6%，从 L4 DRG 的 40.9%增加到 59.1%，从 NG 的 31.6%增加到 38.5%。在发育和衰老过程中，NG 中的 nNOS-IR 神经元百分比没有变化。然而，在 Th2 和 L4 DRG 中，nNOS-IR 神经元的比例在 10 至 30 天龄的动物中较高，并在生命的第二个月下降。在 2 月龄大鼠中，Th2 DRG 中的 nNOS-IR 神经元比例为 52.9%，L4 DRG 中的 nNOS-IR 神经元比例为 51.3%。我们没有发现 Th2 和 L4 DRG 之间以及年轻和老年大鼠之间 nNOS-IR 神经元百分比有统计学差异。在 10 日龄及以上大鼠的 NG 和 DRG 中，高比例的 nNOS-IR 神经元与 isolectin B4 结合。在新生动物中，只有 41.3%、45.3%和 28.4%的 nNOS 神经元在 Th2、L4 DRG 和 NG 中与 IB4 结合。在 10 日龄及以上的大鼠中，与 IB4 结合的感觉 nNOS-IR 神经元数量在 DRG 中达到 90%以上，在 NG 中达到 80%以上。只有少数 nNOS 阳性细胞对降钙素基因相关肽、神经丝 200、钙结合蛋白有免疫反应。这里提供的信息也将为未来研究感觉神经元发育机制提供基础。
Zotero 插件