Mitsuoka Kazuyuki, Miwa Yoko, Kikutani Takeshi, Sato Iwao
Nippon Dental University Hospital, Division of Orthodontics.
Eur J Histochem. 2018 Nov 22;62(4):2976. doi: 10.4081/ejh.2018.2976.
The neuropeptide calcitonin gene-related peptide (CGRP) mediates inflammation and head pain by influencing the functional vascular blood supply. CGRP is a well-characterized mediator of receptor-regulated neurotransmitter release. However, knowledge regarding the role of CGRP during the development of the superior cervical ganglion (SCG) is limited. In the present study, we observed the localization of CGRP and vascular endothelial growth factor (VEGF-A) mRNAs during prenatal development at embryonic day 14.5 (E14.5), E17.5 and postnatal day 1 (P1) using in situ hybridization. The antisense probe for CGRP was detected by in situ hybridization at E14.5, E17.5, and P1, and the highest levels were detected at E17.5. In contrast, the antisense probe for VEGF-A was detected by in situ hybridization in gradually increasing intensity from E14.5 to P1. The differences in the expression of these two markers revealed specific characteristics related to CGRP concentration and release compared to those of VEGF-A during development. The correlation between CGRP and VEGF-A may influence functional stress and the vascular blood supply during prenatal and postnatal development.
神经肽降钙素基因相关肽（CGRP）通过影响功能性血管血液供应来介导炎症和头痛。CGRP是一种特征明确的受体调节神经递质释放的介质。然而，关于CGRP在颈上神经节（SCG）发育过程中的作用的知识有限。在本研究中，我们使用原位杂交技术观察了胚胎第14.5天（E14.5）、E17.5和出生后第1天（P1）产前发育期间CGRP和血管内皮生长因子（VEGF-A）mRNA的定位。在E14.5、E17.5和P1通过原位杂交检测到CGRP的反义探针，在E17.5检测到最高水平。相比之下，通过原位杂交检测到VEGF-A的反义探针从E14.5到P1强度逐渐增加。这两种标记物表达的差异揭示了与发育过程中VEGF-A相比CGRP浓度和释放相关的特定特征。CGRP和VEGF-A之间的相关性可能会影响产前和产后发育期间的功能应激和血管血液供应。
