Kubo K
Anaesthesist. 1985 Oct;34(10):502-7.
The influence of atropine on the release of histamine following the administration of tubocurarine (Amelizol) was studied in anesthetized monkeys. Four groups were prepared: group (a) was not given atropine sulfate as a premedication and (b), (c) and (d) were given atropine 0.0125 mg/kg, 0.025 mg/kg, 0.05 mg/kg by intramuscular injection 30 min before the first blood sampling, respectively. Tubocurarine was given in a dose 0.5 mg/kg intravenously, to all animals. Blood samples were obtained in cases of the preinjection (control) and 1, 3, 6, 10 and 20 min after injection of tubocurarine. The concentration of histamine in the plasma was measured by a fluorometric method and high speed liquid chromatography. When atropine sulfate was given in doses over 0.025 mg/kg intramuscularly 30 min before the administration of tubocurarine, the release of histamine induced by tubocurarine was inhibited, compared to the findings in the non-pretreated groups. Atropine sulfate probably inhibits histamine release induced by tubocurarine through the mediation of intracellular cyclic GMP and atropine can be used as a premedicant in cases of general anaesthesia.
在麻醉的猴子身上研究了阿托品对注射筒箭毒碱（阿美索）后组胺释放的影响。实验设置了四组：（a）组未给予硫酸阿托品作为术前用药，（b）、（c）和（d）组分别在首次采血前30分钟通过肌肉注射给予0.0125mg/kg、0.025mg/kg、0.05mg/kg的阿托品。所有动物均静脉注射0.5mg/kg的筒箭毒碱。在注射筒箭毒碱前（对照）以及注射后1、3、6、10和20分钟采集血样。采用荧光法和高效液相色谱法测定血浆中组胺的浓度。与未预处理组的结果相比，在注射筒箭毒碱前30分钟肌肉注射剂量超过0.025mg/kg的硫酸阿托品时，筒箭毒碱诱导的组胺释放受到抑制。硫酸阿托品可能通过细胞内环鸟苷酸的介导抑制筒箭毒碱诱导的组胺释放，阿托品可作为全身麻醉时的术前用药。
