Lobb R R, Alderman E M, Fett J W
Biochemistry. 1985 Sep 10;24(19):4969-73. doi: 10.1021/bi00340a001.
The angiogenic capacity of the class 1 heparin-binding growth factor from bovine brain, an anionic endothelial cell mitogen of Mr 16 000, has been evaluated. Its ability to induce the growth of new blood vessels has been assessed by means of two established assay systems. On the embryonic chick chorioallantoic membrane dose-response studies demonstrate that 160 ng (10 pmol) of mitogen is required to induce angiogenesis in greater than 50% of the eggs within 72 h. In the presence of 1 unit of exogenous heparin only 40 ng of mitogen (2.5 pmol) is needed to induce a similar response. Moreover, this occurs within 48 h, indicating that heparin also augments the angiogenic response by enhancing the rate of induction of angiogenesis. Eighty nanograms (5 pmol) of mitogen also induces the ingrowth of new blood vessels into the rabbit cornea, both in the presence and in the absence of heparin. These results establish that the class 1 heparin-binding growth factor from bovine brain is an angiogenesis factor. Importantly, the neovascularization induced by this angiogenesis factor is enhanced by heparin. The mechanistic implications for neovascularization under certain normal and pathological conditions are discussed.
已对来自牛脑的1类肝素结合生长因子（一种分子量为16000的阴离子内皮细胞有丝分裂原）的血管生成能力进行了评估。已通过两种既定的检测系统评估了其诱导新血管生长的能力。在胚胎鸡绒毛尿囊膜上进行的剂量反应研究表明，在72小时内，需要160纳克（10皮摩尔）有丝分裂原才能在超过50%的鸡蛋中诱导血管生成。在存在1单位外源性肝素的情况下，仅需40纳克有丝分裂原（2.5皮摩尔）就能诱导出类似的反应。此外，这在48小时内就会发生，表明肝素还通过提高血管生成的诱导速率来增强血管生成反应。80纳克（5皮摩尔）有丝分裂原在有肝素和无肝素的情况下均能诱导新血管长入兔角膜。这些结果表明，来自牛脑的1类肝素结合生长因子是一种血管生成因子。重要的是，这种血管生成因子诱导的新血管形成会被肝素增强。文中讨论了在某些正常和病理条件下新血管形成的机制意义。
