Yassini P R, Stickler D L, Bloomfield S M, Wiggins R C, Konat G W
Department of Anatomy West Virginia University School of Medicine, Morgantown 26506-9128.
Metab Brain Dis. 1994 Dec;9(4):391-9. doi: 10.1007/BF02098885.
Induction of angiogenesis is essential for the continued growth of solid tumors, and one critical component of tumor-induced angiogenesis involves the stimulation of microvascular cells to migrate into the growing mass. We have developed a convenient model system utilizing dual co-culture chambers to study cellular chemotaxis induced by glioma cells in vitro. In this system, rat C6 glioma cells induced migration of fibroblasts and brain capillary endothelial cells. The migratory response was directly related to the number of C6 cells serving as stimulus in the lower chamber. Similar migratory responses were induced by C6 cell conditioned medium in a concentration dependent fashion. Medium conditioned by cultured human anaplastic astrocytoma cells was also found to contain potent chemotactic factor(s). This system may ultimately be employed in the identification of particular glioma cell population(s) and secreted factor(s) responsible for the chemoattraction of microvascular cells.
血管生成的诱导对于实体瘤的持续生长至关重要，而肿瘤诱导血管生成的一个关键组成部分涉及刺激微血管细胞迁移到生长中的肿块中。我们开发了一种利用双共培养室的便捷模型系统，以研究体外胶质瘤细胞诱导的细胞趋化性。在该系统中，大鼠C6胶质瘤细胞诱导成纤维细胞和脑毛细血管内皮细胞迁移。迁移反应与下室中作为刺激物的C6细胞数量直接相关。C6细胞条件培养基以浓度依赖的方式诱导了类似的迁移反应。还发现培养的人间变性星形细胞瘤细胞条件培养基含有有效的趋化因子。该系统最终可用于鉴定负责微血管细胞化学吸引的特定胶质瘤细胞群体和分泌因子。
