Kostrov S V, Izotova L S, Eremashvili M R, Galaktionov K I, Iurin V L
Biokhimiia. 1985 Dec;50(12):2031-9.
Using radioimmunological methods based on the use of mono- and polyclonal antibodies raised against interferon alpha A, it was shown that polyclonal antibodies quantitatively reacted not only with this protein, but also with interferons alpha F and alpha N, whereas all the variants of monoclonal antibodies studied reacted only with interferons alpha A and alpha N. Monoclonal antibodies 5A6, 11E9, 19C10, 258 and 268 are directed against overlapping epitopes of the interferon alpha A molecule, which simultaneously binds not more than two molecules of antibodies with different specificity. The correlation between immunochemical and biological activities of interferon alpha A during temperature denaturation and proteolytic degradation and its ability to form oligomeric complexes were investigated.
运用基于针对α - A干扰素产生的单克隆和多克隆抗体的放射免疫方法，结果显示多克隆抗体不仅能与该蛋白发生定量反应，还能与α - F干扰素和α - N干扰素发生反应，而所研究的所有单克隆抗体变体仅与α - A干扰素和α - N干扰素发生反应。单克隆抗体5A6、11E9、19C10、258和268针对α - A干扰素分子的重叠表位，该分子同时结合的具有不同特异性的抗体分子不超过两个。研究了α - A干扰素在温度变性和蛋白水解降解过程中免疫化学活性与生物学活性之间的相关性及其形成寡聚体复合物的能力。
