Brisson-Lougarre A, Abbal M, Hoff M, Blum C J
C R Acad Sci III. 1985;301(16):727-30.
A high excess of circulating T3 was observed in an euthyroid woman. Agarose gel electrophoresis of serum preincubated with 125I-T3 revealed an abnormal T3-binding in gamma-globulin zone. This binding interfered with the hormone radioimmunoassay. Immunological characterization identified this protein as an IgG-K and IgG-lambda polyclonal antibody that bound T3 but not T4. Scatchard analysis of 125I-T3 binding to the gamma-globulin fraction isolated showed a single class of binding sites with a high affinity Ka = 0.4 X 10(9) L/M and maximal binding capacity of 5.2 X 10(-9) M.
在一名甲状腺功能正常的女性中观察到循环中T3水平显著升高。用125I-T3预孵育的血清进行琼脂糖凝胶电泳显示，在γ-球蛋白区存在异常的T3结合。这种结合干扰了激素放射免疫测定。免疫特性鉴定表明，该蛋白是一种IgG-K和IgG-λ多克隆抗体，它能结合T3但不能结合T4。对分离出的γ-球蛋白部分进行125I-T3结合的Scatchard分析显示，存在一类具有高亲和力（Ka = 0.4×10⁹ L/M）和最大结合容量为5.2×10⁻⁹ M的结合位点。
