Henkel R D, Kennedy R C, Sparrow J T, Dreesman G R
Clin Immunol Immunopathol. 1985 May;35(2):146-55. doi: 10.1016/0090-1229(85)90061-3.
Hepatitis B surface antigen (HBsAg), produced by a human hepatoma which had been transplanted into athymic nude mice, was specifically detected in vivo by 131I-labeled monoclonal antibodies (McAb) directed against distinct epitopes of HBsAg (anti-HBs). Significantly higher levels of radioactivity were present in the hepatoma secreting HBsAg when compared to either a non-HBsAg producing epidermoid tumor or most other tissues obtained from nude mice treated with the 131I-labeled anti-Hbs McAb. A radiolabeled control McAb that did not recognize HBsAg failed to discriminate between either the HBsAg positive and negative tumors or other tissues from nude mice. These data demonstrate the in vivo immunological specificity of anti-HBs McAb for HBsAg associated with a hepatoma tumor.
将人肝癌移植到无胸腺裸鼠体内后产生的乙型肝炎表面抗原（HBsAg），可通过针对HBsAg不同表位的131I标记单克隆抗体（McAb）（抗-HBs）在体内进行特异性检测。与不分泌HBsAg的表皮样肿瘤或用131I标记抗-HBs McAb处理的裸鼠获得的大多数其他组织相比，分泌HBsAg的肝癌中放射性水平显著更高。一种不识别HBsAg的放射性标记对照McAb无法区分HBsAg阳性和阴性肿瘤或裸鼠的其他组织。这些数据证明了抗-HBs McAb对与肝癌相关的HBsAg的体内免疫特异性。
