MacNab G M, Alexander J J, Lecatsas G, Bey E M, Urbanowicz J M
Br J Cancer. 1976 Nov;34(5):509-15. doi: 10.1038/bjc.1976.205.
The human hepatoma cell line, PLC/PRF/5, was shown to produce hepatitis B surface antigen (HBsAg). Immunologically reactive material was present in the supernatant tissue culture medium in significant amounts, and was associated with spherical particles approximately 20 nm in diameter. The rate of antigen production by the cells was estimated at 500 ng/day/10(6) cells by reference to a purified HBsAg standard. All immunological activity was neutralized by specific antibody and the subtype was ad. The studies reported here broaden the scope of investigations on both the in vitro production of HBsAg and the association between this antigen and primary liver cancer.
人肝癌细胞系PLC/PRF/5被证明能产生乙型肝炎表面抗原（HBsAg）。具有免疫反应性的物质大量存在于上清组织培养基中，并与直径约20纳米的球形颗粒相关。参照纯化的HBsAg标准品，细胞产生抗原的速率估计为500纳克/天/10⁶个细胞。所有免疫活性均被特异性抗体中和，亚型为ad。本文报道的研究拓宽了关于HBsAg体外产生以及该抗原与原发性肝癌之间关联的研究范围。
