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The CRISPR/Cas system mediates efficient genome engineering in Bombyx mori.

作者信息

Wang Yueqiang, Li Zhiqian, Xu Jun, Zeng Baosheng, Ling Lin, You Lang, Chen Yazhou, Huang Yongping, Tan Anjiang

机构信息

1] Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China [2] University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Cell Res. 2013 Dec;23(12):1414-6. doi: 10.1038/cr.2013.146. Epub 2013 Oct 29.

Abstract
摘要

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Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.
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ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering.
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One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.
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CRISPR-Cas: evolution of an RNA-based adaptive immunity system in prokaryotes.
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Efficient genome editing in zebrafish using a CRISPR-Cas system.
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Highly efficient and specific genome editing in silkworm using custom TALENs.
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Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori.
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