Department of Renewable Resources, University of Alberta, T6G 2H1, Edmonton, Alberta, Canada.
Theor Appl Genet. 1996 Jan;92(1):46-52. doi: 10.1007/BF00222950.
Fragments of random amplified polymorphic DNA (RAPDs) were used as markers to distinguish Picea glauca (Moench) Voss (white spruce) and Picea engelmannii Parry (Engelmann spruce). These species and their putative hybrids are difficult to differentiate morphologically and are collectively known as interior spruce. Four oligodeoxynucleotide decamer primers showed species-specific amplification products between white spruce and Engelmann spruce. These fragments are highly conserved among seed lots and individual trees of each species from diverse geographic origins. The consistency and reproducibility of these species-specific amplification products were tested in more than two amplification reactions. Therefore, RAPD markers can provide genetic markers for easy and rapid identification of the specific genetic entry of these spruce species and their reported putative hybrids. According to the frequencies of the species-specific RAPD markers, it is possible to estimate the hybrid fraction, indicative of true introgression between the two species. These results are useful for quick identification of both species and their hybrid swarms at any stage in the sporophyte phase of the life cycle, for determining the occurrence and the magnitude of introgressive hybridization in an overlap zone between the two species, and for certification purposes in operational re-forestation and tree-improvement programs.
随机扩增多态性 DNA (RAPD) 片段被用作标记，以区分白云杉（Moench）Voss（白云杉）和恩格尔曼云杉（Parry）（恩格尔曼云杉）。这些物种及其可能的杂种在形态上难以区分，统称为内部云杉。四个寡脱氧核苷酸十聚体引物在白云杉和恩格尔曼云杉之间显示出物种特异性的扩增产物。这些片段在来自不同地理起源的每个物种的种子批和个体树木中高度保守。这些物种特异性扩增产物的一致性和可重复性在两次以上的扩增反应中进行了测试。因此，RAPD 标记可以为这些云杉物种及其报道的可能杂种的特定遗传品系的快速识别提供遗传标记。根据物种特异性 RAPD 标记的频率，可以估计杂种分数，表明两个物种之间存在真正的基因渗入。这些结果对于在生活史孢子体阶段的任何阶段快速识别这两个物种及其杂种群、确定两个物种重叠区渗入杂交的发生和程度以及在操作再造林和树木改良计划中进行认证都是有用的。
