Forest Biotechnology Centre, British Columbia Research Corporation, 3650 Wesbrook Mall, V6S 2L2, Vancouver, B.C., Canada.
Theor Appl Genet. 1991 Aug;82(2):242-8. doi: 10.1007/BF00226220.
The cloning of white spruce (Picea glauca) mitochondrial DNA homologous to the cytochrome oxidase II and ATPaseα genes of maize is described. These probes were used to define restriction fragment length polymorphisms which distinguish the white, Engelmann (P. engelmannii) and Sitka spruce (P. sitchensis) populations that occur in British Columbia. Analysis of progeny from crosses between the species revealed that mitochondrial DNA was maternally inherited in all cases (32 progeny from five independent crosses). The inheritance of chloroplast DNA was determined using a probe described previously; in this case, all progeny exhibited paternal inheritance (27 progeny from four crosses). Mitochondrial and chloroplast probes were used to test trees from zones of introgression between coastal (Sitka) and interior spruces (white and Engelmann). In most cases mitochondria and chloroplasts within individuals were contributed by different species. The data shows that there is a significant Sitka spruce component in trees east of the coastal watershed in British Columbia.
本文描述了克隆白松（Picea glauca）线粒体 DNA 与玉米细胞色素氧化酶 II 和 ATPaseα基因的同源性。这些探针被用来定义限制片段长度多态性，这些多态性可以区分不列颠哥伦比亚省的白松、恩格尔曼（P. engelmannii）和西特卡松（P. sitchensis）种群。对来自物种间杂交的后代的分析表明，线粒体 DNA 在所有情况下都是母系遗传的（来自五个独立杂交的 32 个后代）。叶绿体 DNA 的遗传通过使用先前描述的探针来确定；在这种情况下，所有后代都表现出父系遗传（来自四个杂交的 27 个后代）。线粒体和叶绿体探针被用于测试沿海（西特卡）和内陆松（白松和恩格尔曼）之间杂交区的树木。在大多数情况下，个体内的线粒体和叶绿体来自不同的物种。这些数据表明，不列颠哥伦比亚省沿海流域以东的树木中有一个显著的西特卡松成分。
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