与赋予大豆胞囊线虫（Heterodera glycines）广谱抗性的基因座相关联的 RFLP 标记。
Association of RFLP markers with loci conferring broad-based resistance to the soybean cyst nematode (Heterodera glycines).
机构信息
Indiana Crop Improvement Association and Department of Agronomy, Purdue University, 47907-1150, West Lafayette, IN, USA.
出版信息
Theor Appl Genet. 1996 Jan;92(1):83-6. doi: 10.1007/BF00222955.
Soybean cyst nematode (SCN) is a major soybean yield-limiting pest. The present study was conducted to map broad-based SCN resistance loci from the cultivar 'Hartwig'. Two-hundred F2∶3 lines derived from the cross 'Williams 82' x 'Hartwig' were screened with a fourth-generation SCN inbred and 56 polymorphic molecular markers. Allele states and phenotypes were analyzed using stepwise regression and the model selection was made at P [Symbol: see text] 0.01. Four unlinked RFLP markers (A006, A567, A487, A112) were associated with SCN resistance and the partial coefficient of determinations (R(2)) were 91%, 1%, 1%, and 1%. We have mapped a new, major SCN resistance locus (A006) and three minor loci (A567, A487, A112). This complete mapping will accelerate the transfer of broad-based resistance without linkage drag and aid in the determination of relationships among various SCN-resistant germplasm sources.
大豆胞囊线虫（SCN）是一种严重限制大豆产量的害虫。本研究旨在从品种‘Hartwig’中定位广谱抗 SCN 基因。利用第四代 SCN 近交系和 56 个多态性分子标记，对‘Williams 82’בHartwig’杂交产生的 200 个 F2∶3 代群体进行了筛选。采用逐步回归分析等位基因状态和表型，并在 P [Symbol: see text] 0.01 水平进行模型选择。四个不连锁的 RFLP 标记（A006、A567、A487、A112）与 SCN 抗性相关，部分决定系数（R2）分别为 91%、1%、1%和 1%。我们已经定位到一个新的、主要的 SCN 抗性基因座（A006）和三个次要基因座（A567、A487、A112）。这一完整的图谱将加速无连锁累赘的广谱抗性转移，并有助于确定各种抗 SCN 种质资源之间的关系。
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