Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo de Lellis, 01100, Viterbo, Italy.
Theor Appl Genet. 1996 May;92(6):654-9. doi: 10.1007/BF00226085.
A hexaploid wheat landrace collected from the Baluchistan province of Pakistan was found to possess a novel high-molecular-weight glutenin subunit (HMW-GS). The subunit has a very slow electrophoretic mobility as revealed by SDS-PAGE, and its molecular weight is comparable to that of the highest molecular weight glutenin subunit ("2.2" encoded in the D-genome) reported so far in hexaploid wheat varieties and landraces of Japanese origin. Evidence obtained from (PCR) gene amplification studies using the primers specific for Glu-1 loci proved that the gene coding for this novel subunit belongs to the Glu-A1 locus located on the long arm of chromosome 1A. Digestion of the amplified gene (PCR product) with restriction enzymes indicated that the novel gene differs from prevailing Glu-A1 alleles (null, 1 and 2()) by an extra DNA fragment of approximately 600 base pairs. The results also indicated that the novel subunit is most probably a derivative of subunit 2() that has very likely incorporated the 600-bp fragment following a process of unequal crossing over. The present findings were further substantiated by reserved phase high performance liquid chromatography (RP-HPLC) analysis.
从巴基斯坦俾路支省收集的一个六倍体小麦地方品种被发现具有一种新的高分子量谷蛋白亚基（HMW-GS）。该亚基的 SDS-PAGE 电泳迁移率非常慢，其分子量与迄今为止在六倍体小麦品种和日本起源的地方品种中报道的最高分子量谷蛋白亚基（“2.2”由 D 基因组编码）相当。使用针对 Glu-1 基因座的引物进行（PCR）基因扩增研究获得的证据证明，编码该新亚基的基因属于位于 1A 染色体长臂上的 Glu-A1 基因座。用限制酶消化扩增的基因（PCR 产物）表明，该新基因与流行的 Glu-A1 等位基因（null、1 和 2()) 不同，有大约 600 个碱基对的额外 DNA 片段。结果还表明，该新亚基很可能是亚基 2()的衍生物，很可能在不均等交换过程中掺入了 600bp 片段。保留相高效液相色谱（RP-HPLC）分析进一步证实了这一发现。
