Citrus Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 700 Experiment Station Road, 33850, Lake Alfred, FL, USA.
Theor Appl Genet. 1996 May;92(6):688-95. doi: 10.1007/BF00226090.
A localized genetic linkage map was developed of the region surrounding the citrus tristeza virus (CTV) resistance gene (designated Ctv) from Poncirus trifoliate L., a sexually compatible Citrus relative. Bulked segregant analysis (BSA) was used to identify potential resistance-associated RAPD fragment markers in four intergeneric backcross families that were segregating for CTV resistance. Eight RAPD fragments were found that were consistently linked to Ctv in the four families. Map distances and locus order were determined with MAPMAKER 3.0, using the results obtained from 59 individuals in the largest family. Also, a consensus map was constructed with JOINMAP 1.3, using pooled results from the four backcross families. Marker orders were identical, except for 1 marker, on these independently developed maps. Family-specific resistance-associated markers were also identified, as were numerous susceptibility-associated markers. The identification of markers tightly linked to Ctv will enable citrus breeders to identify plants likely to be CTV-resistant by indirect, marker-assisted selection, rather than by labor-intensive direct challenge with the pathogen. These markers also provide a basis for future efforts to isolate Ctv for subsequent genetic manipulation.
一个本地化的遗传连锁图谱是开发的柑橘衰退病毒（CTV）抗性基因（命名为 Ctv）从枳属，一个有性亲和柑橘属相关的地区。混池分离分析（BSA）用于鉴定在四个属间回交群体中潜在的与 CTV 抗性相关的 RAPD 片段标记，这些群体正在分离 CTV 抗性。在四个家系中发现了 8 个与 Ctv 一致连锁的 RAPD 片段。利用 MAPMAKER 3.0，根据最大家系中 59 个人的结果，确定了图谱距离和基因座顺序。此外，还利用 JOINMAP 1.3，根据四个回交群体的汇总结果构建了一个共识图谱。这些独立开发的图谱的标记顺序是相同的，除了 1 个标记。还鉴定了与家族特异性抗性相关的标记，以及许多与易感性相关的标记。与 Ctv 紧密连锁标记的鉴定将使柑橘育种者能够通过间接的、基于标记的选择来识别可能具有 CTV 抗性的植物，而不是通过费力的直接病原体挑战。这些标记也为未来分离 Ctv 进行遗传操作提供了基础。
