Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental INIA Salto Grande. Camino al Terrible, Salto, CP 50000, Uruguay.
Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR Litoral Norte), Universidad de la República, Rivera 1350, Salto, CP 50000, Uruguay.
Arch Virol. 2023 Mar 29;168(4):123. doi: 10.1007/s00705-023-05749-y.
Resistance-breaking (RB) isolates of citrus tristeza virus (CTV) can replicate and move systemically in Poncirus trifoliata, a rootstock widely used for management of decline caused by CTV and other purposes. In Uruguay, severe CTV isolates are prevalent, and an RB isolate (designated as RB-UY1) was identified. In order to predict the implications of this genotype circulating in citrus crops grafted on trifoliate rootstocks, the aim of this work was to determine the biological and molecular characteristics of this isolate, the efficiency of its transmission by Toxoptera citricida, and its effects on plant growth performance of P. trifoliata. Our results show that RB-UY1 can be classified as a mild isolate, that it is phylogenetically associated with the RB1 group, and that it is efficiently transmitted by T. citrida. They also suggest that the RB-UY1 isolate should not affect the performance of citrus crops grafted on trifoliate rootstocks, although some growth parameters of P. trifoliata seedlings were affected four years after inoculation.
抗裂果（RB）分离株的柑橘衰退病毒（CTV）可以在枳（Poncirus trifoliata）中复制和系统移动，枳是一种广泛用于管理 CTV 和其他原因引起的衰退的砧木。在乌拉圭，严重的 CTV 分离株普遍存在，并且鉴定出了一个 RB 分离株（称为 RB-UY1）。为了预测这种在嫁接在三叶砧木上的柑橘作物中循环的基因型的影响，本工作的目的是确定该分离株的生物学和分子特征、其在橘蚜（Toxoptera citricida）传播中的效率及其对枳生长性能的影响。我们的结果表明，RB-UY1 可归类为温和分离株，它在系统发育上与 RB1 组相关，并且能够被橘蚜有效地传播。它们还表明，RB-UY1 分离株不应影响嫁接在三叶砧木上的柑橘作物的性能，尽管在接种四年后，枳实生苗的一些生长参数受到了影响。
