Istituto Sperimentale per la Cerealicoltura, Sezione di Bergamo, via Stezzano 24, 24126, Bergamo, Italy.
Theor Appl Genet. 1996 May;92(6):696-701. doi: 10.1007/BF00226091.
Thirty-four accessions from Zea and 10 accessions from related genera were assayed for the presence of Bg, a transposable element originally found in maize (Zea mays ssp. mays). Bg-like sequences, identified as hybridizing bands on Southern blots, were visualized in all Zea accessions and were present in approximately equal numbers in teosinte and maize. With the exception of Tripsacum dactyloides, all accessions from related genera failed to hybridize with the Bg probes, even at reduced stringency. A comparison of the restriction patterns of related inbred lines revealed numerous common hybridizing fragments. An index of molecular similarity (MS) was used to determine the degree of similarity between pairs of inbred lines. Computed MS values endorse an inbred relationship and are in good agreement with published results of cluster analysis on these inbred lines.
对 34 份 Zea 属材料和 10 份相关属材料进行了 Bg 检测，Bg 是一个最初在玉米（Zea mays ssp. mays）中发现的可移动元件。Southern 印迹杂交显示，所有 Zea 属材料中都存在 Bg 类似序列，并且在 teosinte 和玉米中的数量大致相等。除了 Tripsacum dactyloides 之外，所有相关属的材料都未能与 Bg 探针杂交，即使在降低严格性的条件下也未能杂交。对相关自交系限制图谱的比较揭示了许多共同的杂交片段。采用分子相似性指数（MS）来确定自交系之间的相似程度。计算出的 MS 值支持自交关系，并且与这些自交系聚类分析的发表结果非常吻合。
