Department of Botany, University of Delhi, 110 007, Delhi, India.
Theor Appl Genet. 1996 May;92(6):786-90. doi: 10.1007/BF00226103.
Three intergeneric hybrids were produced between a cold-tolerant wild species, Erucastrum abyssinicum and three cultivated species of Brassica, B. juncea, B. carinata and B. oleracea, through ovary culture. The hybrids were characterized by morphology, cytology and DNA analysis. Amphiploidy was induced in all the F1 hybrids through colchicine treatment. Stable amphiploids and backcross progenies were obtained from two of the crosses, E. abyssinicum x B. juncea and E. abyssinicum x B. carinata. The amphiploid, E. abyssinicum x B. juncea was successfully used as a bridge species to produce hybrids with B. napus, B. campestris and B. nigra. These hybrids and backcross progenies provide useful genetic variability for the improvement of crop brassicas.
通过子房培养，在一个耐寒野生种埃塞俄比亚冰菜（Erucastrum abyssinicum）和三个甘蓝型作物品种芥菜（B. juncea）、油白菜（B. carinata）和结球甘蓝（B. oleracea）之间产生了三个属间杂种。杂种通过形态学、细胞学和 DNA 分析进行了鉴定。所有 F1 杂种通过秋水仙碱处理诱导形成了同源多倍体。在埃塞俄比亚冰菜 x 芥菜和埃塞俄比亚冰菜 x 油白菜这两个杂交中，获得了稳定的同源多倍体和回交后代。同源多倍体埃塞俄比亚冰菜 x 芥菜成功地被用作桥梁物种，与油菜（B. napus）、白菜（B. campestris）和黑芥（B. nigra）产生杂种。这些杂种和回交后代为改良作物甘蓝型作物提供了有用的遗传变异。
