用于鉴定种间体细胞杂种的种特异性重复 DNA。
Species-specific repetitive DNA used to identify interspecific somatic hybrids.
机构信息
Friedrich Miescher-Institut, P.O. Box 2543, CH-4002, Basel, Switzerland.
出版信息
Plant Cell Rep. 1984 Apr;3(2):65-7. doi: 10.1007/BF00270973.
PMID:24253395
Abstract
Plasmid DNA clones containing repetitive DNA sequences were isolated from Hyoscyamus muticus and Nicotiana tabacum. Non cross-hybridizing probes from each species were used in a simple hybridization test with DNA isolated from presumptive somatic hybrids. This allowed unequivocal identification of DNA from both species in the hybrids.
摘要
从茄科天仙子属植物（Hyoscyamus muticus）和烟草属植物（Nicotiana tabacum）中分离出含有重复 DNA 序列的质粒 DNA 克隆。来自每个物种的非交叉杂交探针在与假定体细胞杂种分离的 DNA 进行的简单杂交试验中被使用。这使得杂种中来自两个物种的 DNA 能够被明确识别。
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