Demirdögen B, Bonilla C E Plazas, Trujillo S, Perilla J E, Elcin A E, Elcin Y M, Ribelles J L Gómez
Ankara University, Stem Cell Institute, TEBN Laboratory, Ankara, Turkey; Department of Chemistry, Ankara University, Faculty of Science, TEBN Laboratory, Ankara, Turkey.
J Biomed Mater Res A. 2014 Sep;102(9):3229-36. doi: 10.1002/jbm.a.34999. Epub 2013 Oct 25.
Polycaprolactone/silica microporous hybrid membranes were produced in two steps: A microporous polycaprolactone membrane with an interconnected porosity of 80% was obtained via a freeze extraction procedure, then silica was formed by a sol-gel reaction inside the micropores using tetraethyl orthosilicate, TEOS, as silica precursor. It is shown that silica forms a thin coating layer homogeneously distributed over the pore walls when sol-gel reaction is catalyzed by hydrochloric acid, while it forms submicron spherical particles when using basic catalyzer. Some physical properties and the viability and osteoblastic differentiation of bone marrow rat mesenchymal stem cells cultured on pure and hybrid membranes were studied.
聚己内酯/二氧化硅微孔杂化膜分两步制备：通过冷冻萃取法获得孔隙率为80%且孔隙相互连通的微孔聚己内酯膜，然后使用正硅酸乙酯（TEOS）作为二氧化硅前驱体，通过溶胶 - 凝胶反应在微孔内部形成二氧化硅。结果表明，当用盐酸催化溶胶 - 凝胶反应时，二氧化硅在孔壁上形成均匀分布的薄涂层，而使用碱性催化剂时则形成亚微米级球形颗粒。研究了在纯膜和杂化膜上培养的大鼠骨髓间充质干细胞的一些物理性质、活力和成骨分化情况。
