Müller C, Herbst H, Uchanska-Ziegler B, Ziegler A, Schunter F, Steiert I, Muller C, Wernet P
Hum Immunol. 1985 Dec;14(4):333-49. doi: 10.1016/0198-8859(85)90241-1.
The production and serologic, as well as immunochemical properties of a cytotoxic murine IgG monoclonal antibody (Tü109) that precipitates HLA-class I molecules, are described. In the microcytotoxicity assay Tü109 supernatant was demonstrated on a panel of 424 HLA-ABC, -DR, -DQ, -MT typed normal Caucasian blood donors to define an epitope on HLA-B locus molecules in great association with the supertypic specificity Bw4. Reactivity of supernatant showed MHC linked inheritance of the Tü109 determinant and discriminated the HLA-Bw4/Bw6 associated HLA-B locus split antigens. Weak or lack of binding on lymphocytes from some HLA-Bw4 heterozygous individuals, particularly typing for HLA-Bw44, appeared to be due to qualitative and/or quantitative variations of HLA-B locus molecules on the cell surface. With Tü109 ascites fluid, however, extra-reactivity on all HLA-Bw6+ cells was demonstrated. Preferential binding of supernatant to HLA-Bw4, but reactivity of ascites fluid with HLA-Bw6+ molecules in addition, was furthermore confirmed by IEF analysis of antigens immunoprecipitated with Tü109 from cell lysates. Thus the antibody may help to analyze the evolutionary relationship of the diallelic specificities Bw4 and Bw6.
本文描述了一种能沉淀HLA - I类分子的细胞毒性小鼠IgG单克隆抗体（Tü109）的产生、血清学特性以及免疫化学特性。在微量细胞毒性试验中，Tü109上清液在424名HLA - ABC、- DR、- DQ、- MT分型的正常高加索献血者中进行检测，以确定与超型特异性Bw4密切相关的HLA - B位点分子上的一个表位。上清液的反应性显示Tü109决定簇具有MHC连锁遗传特性，并区分了与HLA - Bw4 / Bw6相关的HLA - B位点分裂抗原。一些HLA - Bw4杂合个体的淋巴细胞，尤其是HLA - Bw44分型的淋巴细胞，出现弱结合或无结合现象，这似乎是由于细胞表面HLA - B位点分子的质量和/或数量变化所致。然而，使用Tü109腹水时，在所有HLA - Bw6 +细胞上均显示出额外的反应性。通过对用Tü109从细胞裂解物中免疫沉淀的抗原进行IEF分析，进一步证实了上清液对HLA - Bw4的优先结合以及腹水与HLA - Bw6 +分子的反应性。因此，该抗体可能有助于分析双等位基因特异性Bw4和Bw6的进化关系。
