Wilson D B
J Craniofac Genet Dev Biol. 1985;5(4):363-71.
The neural basal lamina in hindbrain regions of exencephalic loop-tail (Lp/Lp) mice and of their normal (+/+; Lp/+) littermates was analyzed histochemically at the electron microscopic level by means of enzyme digestion and alcian blue staining with critical electrolyte concentrations (CEC) of MgCl2. At 9 days of gestation, the normal and abnormal embryos showed a similar pattern of alcian blue staining with a CEC of 0.00 M or 0.05 M MgCl2. However, with a CEC of 0.30 M MgCl2, the basal lamina in the abnormals stained more prominently, particularly the lamina rara externa, suggesting the presence of more sulfated glycosaminoglycans (GAG) in the abnormals. Moreover, predigestion of the tissues with Streptomyces hyaluronidase, which removes hyaluronic acid (HA), indicated that the abnormal basal lamina contained relatively less HA than in the normal embryos. By 10 days of gestation the normal basal lamina contained relatively more sulfated GAG and less HA and was thus more similar in appearance to that in the abnormal embryos. This apparently premature shift from HA predominance to sulfated GAG predominance in the abnormal basal lamina may be of significance in the etiology of dysraphism in this mutant.
通过酶消化和用关键电解质浓度（CEC）的氯化镁进行阿尔辛蓝染色，在电子显微镜水平对无脑回环尾（Lp/Lp）小鼠及其正常（+/+；Lp/+）同窝仔鼠后脑区域的神经基底膜进行了组织化学分析。在妊娠第9天，正常和异常胚胎在CEC为0.00 M或0.05 M氯化镁时显示出相似的阿尔辛蓝染色模式。然而，在CEC为0.30 M氯化镁时，异常胚胎的基底膜染色更明显，尤其是外疏松层，表明异常胚胎中存在更多的硫酸化糖胺聚糖（GAG）。此外，用能去除透明质酸（HA）的透明质酸酶对组织进行预消化表明，异常基底膜中的HA含量比正常胚胎相对较少。到妊娠第10天，正常基底膜含有相对更多的硫酸化GAG和更少的HA，因此在外观上与异常胚胎的基底膜更相似。这种异常基底膜中从HA占主导地位到硫酸化GAG占主导地位的明显过早转变可能在该突变体神经管闭合不全的病因学中具有重要意义。
