Narayanan R B, Ramu G, Sinha S, Sengupta U, Malaviya G N, Desikan K V
Indian J Lepr. 1985 Apr-Jun;57(2):258-64.
Cryostat sections of dermal lesions from 13 untreated patients of leprosy were studied by indirect immunoperoxidase using monoclonal antibodies (MLO4 & MLO6), defining M. leprae specific antigens. The lymphocytes and macrophages in both the tuberculoid and lepromatous granulomas showed membranous staining with the above antibodies. M. leprae organisms in the lepromatous granulomas and the cells in the section of lymph nodes of patients with tuberculosis, or sections of normal skin or psoriatic lesions did not show any staining with these antibodies. These observations suggest that M. leprae specific antigens are present and expressed on the cells infiltrating the granulomas of leprosy lesions.
采用单克隆抗体（MLO4和MLO6）通过间接免疫过氧化物酶法对13例未经治疗的麻风病患者皮肤病变的冰冻切片进行研究，这些抗体可识别麻风杆菌特异性抗原。结核样型和瘤型肉芽肿中的淋巴细胞和巨噬细胞均显示出上述抗体的膜状染色。瘤型肉芽肿中的麻风杆菌以及结核病患者淋巴结切片、正常皮肤切片或银屑病病变切片中的细胞均未显示出这些抗体的任何染色。这些观察结果表明，麻风杆菌特异性抗原存在于麻风病病变肉芽肿浸润细胞上并在这些细胞上表达。
